Research Advance on miRNA qPCR Methods and Its Application

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.008

Abstract

Abstract: miRNAis one of thehottestresearch fields in the world.The detection of miRNA expression is the fundamental content in miRNA research.Due to the feature of miRNAs being short and small, varied methods were developed, and quantitative miRNA qPCR isthe most widely used one.This paper reviews thedevelopments of miRNA extraction method, quantitative detection principle of miRNAqPCR, primer design, the reaction condition, and reference gene selection.The developments ofmiRNA qPCR methods in the future are also discussed.All of these may provide a reference for researchers in the field when they detect miRNA by qPCR method.

Key words: miRNA, stem-loop RT-PCR, poly(A)tailing RT-PCR, extension RT-PCR, digital PCR

FENG Shi-peng. Research Advance on miRNA qPCR Methods and Its Application[J]. Biotechnology Bulletin, 2016, 32(2): 59-69.

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