Improving the Percentage of Exerted Stigma in CMS Lines of Japonica Hybrid Rice by Molecular Marker-assisted Selection

doi:10.13560/j.cnki.biotech.bull.1985.2016.03.009

Biotechnology Bulletin ›› 2016, Vol. 32 ›› Issue (3): 52-57.doi: 10.13560/j.cnki.biotech.bull.1985.2016.03.009

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Improving the Percentage of Exerted Stigma in CMS Lines of Japonica Hybrid Rice by Molecular Marker-assisted Selection

CAI Zhi-jun¹, LI Jin-jun¹, ZHOU De-yin², FU Hao-wei¹

1. Jiaxing Academy of Agricultural Science Research of Zhejiang Province, Jiaxing 314016；
2. Agricultural Commission of Xinghua City in Jiangsu Province, Xinghua 225700

Received:2015-10-06 Online:2016-03-24 Published:2016-03-25

Abstract

Abstract: It is the most effective way to improve the yield of seed production of Japonica hybrid rice by enhancing the percentage of exerted stigma in its CMS lines. In this research, QTLs(qPES3, qPES9, qPES12), regulating the percentage of exerted stigma in indica rice, were transmitted into CMS lines of Japonica hybrid rice through crossing and backcrossing, aiming at selecting the CMS lines of Japonica hybrid rice with high percentage of exerted stigma by pedigree breeding and molecular detection technique, i.e., exploring the feasibility of improving the percentage of exerted stigma in CMS lines of Japonica hybrid rice by molecular marker technique. The research results showed that Jialong17A and Jialong43A, two CMS lines developed in this study, carried both qPES3and qPES12. The phenotypic values of percentage of exerted stigma and outcrossing rate indicated that QTLs expressed in Japonica rice, but displaying difference in various genetic background. Conclusively, molecular marker-assisted selection(MAS)is an efficient method to improve the percentage of exerted stigma in CMS lines of Japonica hybrid rice.

Key words: CMS lines of Japonica hybrid rice, percentage of exerted stigma, molecular marker-assisted selection

CAI Zhi-jun, LI Jin-jun, ZHOU De-yin, FU Hao-wei. Improving the Percentage of Exerted Stigma in CMS Lines of Japonica Hybrid Rice by Molecular Marker-assisted Selection[J]. Biotechnology Bulletin, 2016, 32(3): 52-57.

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Improving the Percentage of Exerted Stigma in CMS Lines of Japonica Hybrid Rice by Molecular Marker-assisted Selection

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