Research Advances on Rapid Detection Methods for Aflatoxin Based on Biological Binders

doi:10.13560/j.cnki.biotech.bull.1985.2016.08.009

Abstract

Abstract: Aflatoxins are secondary metabolites secreted by Aspergillus flavus and Aspergillus parasiticus,easily pollute the grains and their processed products,and mainly exist in moldy grain,legumes,nuts and associated food products. Aflatoxins are extremely toxic and carcinogenic,especially aflatoxin B1 that has been found to be the strongest natural carcinogen and severely threat to human health. Current detection methods of aflatoxins in the grains are mainly instrumental analysis by combining the high performance liquid chromatography and liquid mass spectrometry,however,instrumental analysis presents the shortcomings such as instruments cost is very high,analysis process is very complex and needs long-time-trained professionals. In recent years,integrating the rapid identification of aflatoxin based on biological binders（antibody,recombinant antibody,and aptamer）and different signal reporters,a series of analysis methods for aflatoxin were established,and they are fast,flexible,and easy to operate,therefore widely applied in food safety. Here we review the rapid detection methods mainly from 2 aspects of specific biological binders for aflatoxins and signal reporters,compare and evaluate them,also prospect the development trend of rapid methods for aflatoxin.

Key words: aflatoxin, antibody, aptamer, rapid detection

ZENG Kun, DU Dao-lin, XUE Yong-lai. Research Advances on Rapid Detection Methods for Aflatoxin Based on Biological Binders[J]. Biotechnology Bulletin, 2016, 32(8): 47-55.

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