Progress in Selection and Application of Antibacterial Aptamers

doi:10.13560/j.cnki.biotech.bull.1985.2022-0860

Abstract

Abstract:

One of the major challenges in modern medicine and molecular biotechnology is to explore new therapeutic options for bacterial infections. The increasing rates of multidrug-resistant bacteria, overlaid with the efficacy, development process, and cost of antimicrobial agents, has made the treatments of bacterial infections even more challenging. In response to this situation, the most promising solution is to find alternative sources of antibacterial drugs, and antibacterial aptamers obtained through SELEX screening are promising alternatives. Antibacterial aptamers can reduce the pathogenic abilities of bacteria by interfering with the biochemical processes of bacteria, controlling the formations of bacterial membrane and blocking the infections of toxins. Currently, aptamer-based antibacterial strategies mainly include single aptamer antibacteria, aptamer composite nanomaterials, aptamer composite antibiotics, etc. In this paper, the screening strategies of aptamers for common pathogenic cells or their related components are summarized, and the adaptation and modification of aptamers are discussed from the aspects of antimicrobial mechanism and its application in antimicrobial therapy. The development prospect of antimicrobial strategies based on antibacterial aptamers in the treatment of infection is prospected, aiming to provide new ideas for the diagnosis and treatment of infectious diseases caused by pathogenic bacteria.

Key words: antibacterial aptamer, selection, bacteriostatic mechanism

LI Dian-dian, SU Yuan, LI Jie, XU Wen-tao, ZHU Long-jiao. Progress in Selection and Application of Antibacterial Aptamers[J]. Biotechnology Bulletin, 2023, 39(6): 126-132.

Figures/Tables 3

References 38

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常见致病菌 Common pathogenic bacterium	菌型 Bacterial type	靶标 Target	适配体（类型） Aptamer（type）	序列 Sequence（5'-3'）	Kd/ （nmol·L^-1）	参考文献 Reference
沙门氏菌 Salmonellae	鼠伤寒沙门氏菌 S. typhimurium	IVB型菌毛 Type IVB pili	S-PS_8.4（RNA）	UCACUGUUAUCCGAUAGCAGCGCGGGAUGA	8.56	[7]
	鼠伤寒沙门氏菌 S. typhimurium	全细胞 Whole cells	ST-12, ST-33（DNA）	CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGT, CTCCTCTGACTGTAACCACGGTGGGAGAGATGCTATACAATCTTGTAAGGCGATGGACCG	4.5±0.4, 51.0±4.3	[8]
	肠炎沙门氏菌 S. enteritidis	SipA蛋白 SipA protein	Apt17（DNA）	TAGGGAAGAGAAGGACATATGATGCAATGGAACCGCTGAACGACCCTAG CATTATCAGTGTGGTTGACTAGTACATGACCACTTGA	114.9（27℃）, 63.4（37℃）	[9]
	猪霍乱沙门氏菌 S. choleraesuis	鞭毛蛋白 Flagellin	Aptamer 3（DNA）	GGCAGGACAACAGCGTGTAGTATCAGCTTACGGTG	41.0±2.0	[10]
结核分枝杆菌 Mycobacterium tuberculosis	H37Rv	PPK2	G9（DNA）	AACACATAGGTTTGGTTAGGTTGGTTGGTTGAATTA	870±220	[11]
结核分枝杆菌 Mycobacterium tuberculosis	H37Rv	全细胞 Whole cells	MS10-Trunc（DNA）	GGTGTGTTGACTGAGGGGGTGGGGTGGGTGGTGGTGGATATAGC	0.019	[12]
铜绿假单胞菌 Pseudomonas aeruginosa		C4-HSL	A16（DNA）	CCATCCACACTCCGCAAGTGGGGAGGGGAGAGACGACGATCCTGTGGGT TTTCTGCAGTGAGTCGTGTTTTCGACTTATTGCGTCGGCTGCCTCTACAT	28.47	[13]
金黄色葡萄球菌 Staphylococcus aureus		肠毒素A Enterotoxin A	S3（DNA）	CCCGCCTCTGAGCATTATTAATGTTATACCTTACGGCTGG	36.93±7.29	[14]

常见致病菌 Common pathogenic bacterium	菌型 Bacterial type	靶标 Target	适配体（类型） Aptamer（type）	序列 Sequence（5'-3'）	Kd/ （nmol·L^-1）	参考文献 Reference
沙门氏菌 Salmonellae	鼠伤寒沙门氏菌 S. typhimurium	IVB型菌毛 Type IVB pili	S-PS_8.4（RNA）	UCACUGUUAUCCGAUAGCAGCGCGGGAUGA	8.56	[7]
	鼠伤寒沙门氏菌 S. typhimurium	全细胞 Whole cells	ST-12, ST-33（DNA）	CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGT, CTCCTCTGACTGTAACCACGGTGGGAGAGATGCTATACAATCTTGTAAGGCGATGGACCG	4.5±0.4, 51.0±4.3	[8]
	肠炎沙门氏菌 S. enteritidis	SipA蛋白 SipA protein	Apt17（DNA）	TAGGGAAGAGAAGGACATATGATGCAATGGAACCGCTGAACGACCCTAG CATTATCAGTGTGGTTGACTAGTACATGACCACTTGA	114.9（27℃）, 63.4（37℃）	[9]
	猪霍乱沙门氏菌 S. choleraesuis	鞭毛蛋白 Flagellin	Aptamer 3（DNA）	GGCAGGACAACAGCGTGTAGTATCAGCTTACGGTG	41.0±2.0	[10]
结核分枝杆菌 Mycobacterium tuberculosis	H37Rv	PPK2	G9（DNA）	AACACATAGGTTTGGTTAGGTTGGTTGGTTGAATTA	870±220	[11]
结核分枝杆菌 Mycobacterium tuberculosis	H37Rv	全细胞 Whole cells	MS10-Trunc（DNA）	GGTGTGTTGACTGAGGGGGTGGGGTGGGTGGTGGTGGATATAGC	0.019	[12]
铜绿假单胞菌 Pseudomonas aeruginosa		C4-HSL	A16（DNA）	CCATCCACACTCCGCAAGTGGGGAGGGGAGAGACGACGATCCTGTGGGT TTTCTGCAGTGAGTCGTGTTTTCGACTTATTGCGTCGGCTGCCTCTACAT	28.47	[13]
金黄色葡萄球菌 Staphylococcus aureus		肠毒素A Enterotoxin A	S3（DNA）	CCCGCCTCTGAGCATTATTAATGTTATACCTTACGGCTGG	36.93±7.29	[14]