Screening for the Interaction Proteins of SOC1 from Phyllostachys violascens Using Yeast Two-Hybrid System

doi:10.13560/j.cnki.biotech.bull.1985.2016.08.030

Abstract

Abstract: This work aims to study the target proteins of suppressor of overexpression of constans1（SOC1）in flowering way of Phyllostachys violaceus and to explore the action mechanism of SOC1. The pGBKT7-PvSOC1 vector was constructed as the bait protein,then the cDNA library of P. violascens flowering was established using SMART technology,and the interaction proteins with SOC1 were screened by yeast two-hybrid system as well as analyzed and identified. The results showed that the cDNA library for yeast two-hybrid was constructed successfully,the conversion rate of the library was about 3.0×10⁶ converters per μg pGADT7-Rec,and the capacity of the library was up to 7.5×10⁶cfu/mL and the insert fragments were distributed from 0.25 kb to 2 kb. The protein interacted with the bait protein PvSOC1 was screened through the yeast two-hybrid technology. The screened target protein was identified as a protein kinase containing plentiful leucine which amino acid sequence was very conservative in different species,and had the highest affinity with rice.

Key words: Phyllostachys violascens, SOC1, cDNA library, yeast two-hybrid, protein interaction

PAN Xian-fei ,SHI Quan, LIN Xin-chun, XU Ying-wu ,CAO You-zhi. Screening for the Interaction Proteins of SOC1 from Phyllostachys violascens Using Yeast Two-Hybrid System[J]. Biotechnology Bulletin, 2016, 32(8): 207-213.

References

[1] Moon J, Suh SS, Lee H, et al. The SOC1 MADS‐box gene integrates vernalization and gibberellin signals for flowering in Arabidopsis[J]. The Plant Journal, 2003, 35（5）：613-623.
[2] 张则婷, 学宝. MADS-box基因在植物发育中的功能[J]. 植物生理学通讯, 2007, 43（2）：218-222.
[3] Ramanayake S. Flowering in bamboo：an enigma[J]. Cey J Sci（Bio Sci）, 2006, 35（2）：95-105.
[4] 林新春, 袁晓亮, 林绕, 等. 雷竹开花生物学特性研究[J]. 福建林学院学报, 2010（4）：333-337.
[5] Fukao Y. Protein-protein interactions in plants[J]. Plant and Cell Physiology, 2012, 53（4）：617-625.
[6] Fields S, Song O. A novel genetic system to detect protein-protein interactions. [J]. Nature, 1989, 340（6230）：245-246.
[7] Ito T, Chiba T, Ozawa R, et al. A comprehensive two-hybrid analysis to explore the yeast protein interactome[J]. Proceedings of the National Academy of Sciences, 2001, 98（8）：4569-4574.
[8] James P, Halladay J, Craig EA. Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast[J]. Genetics, 1996, 144（4）：1425-1436.
[9] Costa M, Nobre MS, Becker JD, et al. Expression-based and co-local-ization detection of arabinogalactan protein 6 and arabinogalactan protein 11 interactors in Arabidopsis pollen and pollen tubes[J]. BMC Plant Biology, 2013, 13（1）：7.
[10] Singh R, Lee MO, Lee JE, et al. Rice mitogen-activated protein kin-ase interactome analysis using the yeast two-hybrid system[J]. Plant Physiology, 2012, 160（1）：477-487.
[11] 崔红军, 张军杰, 黄玉碧. 玉米根部酵母双杂交cDNA文库的构建及评价[J]. 分子植物育种, 2008, 6（1）：161-164.
[12] 张学文, 洪亚辉, 赵燕. 植物开花时期的分子控制[J]. 湖南农业大学学报：自然科学版, 2004, 29（6）：523-528.
[13] 刘丽娜, 刘伟, 叶庆生. 春化作用相关基因FLC的研究进展[J]. 西北植物学报, 2004, 23（12）：2229-2234.
[14] 汤青林, 许俊强, 宋明, 等. 芥菜开花信号整合子的两个核心转录因子FLC和SVP相互作用的体外检测[J]. 园艺学报, 2011, 38（12）：2317-2324.
[15] 张婧. 高等植物开花基因 FT 研究进展[J]. 现代农业科技, 2012（3）：31-32.
[16] Sarma VV. Flowering of Melocanna baccifera（Bambusaceae）in northeastern India[J]. Curr Sci, 2009, 96（9）：1165.
[17] Lin CS, Lin CC, Chang WC. Effect of thidiazuron on vegetative tissue-derived somatic embryogenesis and flowering of bamboo Bambusa edulis[J]. Plant Cell, Tissue and Organ Culture, 2004, 76（1）：75-82.
[18] Chen J, Carter MB, Edwards BS, et al. High throughput flow cytometry based yeast two-hybrid array approach for large-scale analysis of protein-protein interactions[J]. Cytometry Part A, 2012, 81（1）：90-98.
[19] Mulekar JJ, Bu Q, Chen F, et al. Casein kinase II α subunits affect multiple developmental and stress-responsive pathways in Arabidopsis[J]. The Plant Journal, 2012, 69（2）：343-354.
[20] Lu S X, Liu H, Knowles SM, et al. A role for protein kinase casein kinase2 a-subunits in the Arabidopsis circadian clock1[J]. Plant Physiol, 2011, 157（3）：1537.
[21] Kimura Y, Aoki S, Ando E, et al. A flowering integrator, SOC1, affects stomatal opening in Arabidopsis thaliana[J]. Plant and Cell Physiology, 2015, 56（4）：640-649.