Eukaryotic Expression of BMPR1B in Sheep and Identification of Its Interaction Proteins

doi:10.13560/j.cnki.biotech.bull.1985.2019-0447

Abstract

Abstract: This work aims to study the roles and pathways of BMPR1B protein and its interactive protein in the development and ovulation of sheep ovarian oocyte. By constructing the eukaryotic expression system of BMPR1B gene and characterizing BMPR1B protein,CoIP-MS technology was used to identify the specifically interactive proteins with BMPR1B in ewe ovarian extract,and the target protein interaction network was established. Then we used bioinformatics to analyze and predict the interaction pathways of BMPR1B protein and its interaction proteins in the development and ovulation of sheep ovarian oocyte. There were 23 proteins from ewes ovary extract that were correlated with BMPR1B,while 6 target proteins（BMP2,BMP4,GDF5,GDF9,RhoD and HSP10）specifically interacted with BMPR1B. Bioinformatics analysis showed that the target protein constituted complex and close interactions with TGF-β signal transduction pathway,ovarian steroid generation pathway and MAPK signal transduction pathway,and the metabolic pathways of BMPR1B protein regulating the development and ovulation of sheep oocytes was designed based on the results of their interactions. BMPR1B protein interacts with Smads family proteins in lambing trait pathway and plays an important role in the development and ovulation of sheep oocyte.

Key words: ewes, BMPR1B, protein-protein interactions, CoIP-MS, bioinformatics

JIA Jian-lei, CHEN Qian, JIN Ji-peng, YUAN Zan, ZHANG Li-ping. Eukaryotic Expression of BMPR1B in Sheep and Identification of Its Interaction Proteins[J]. Biotechnology Bulletin, 2019, 35(12): 94-104.

References

[1] Zhang X, Li W, Wu Y, et al.Disruption of the sheep BMPR1B gene by CRISPR/Cas9 in in vitro produced embryos[J]. Theriogenology, 2017, 91:163-172.
[2] 张利平. BMPR-IB基因和PGR基因作为小尾寒羊多胎候选基因的研究[D]. 兰州:甘肃农业大学, 2005.
[3] Galloway SM, McNatty KP, Cambridge LM, et al. Mutations in an oocyte-derived growth factor gene(BMP15)cause increased ovulation rate and infertility in a dosage-sensitive manner[J]. Nat Genet, 2000, 25(3):279-283.
[4] 储明星, 狄冉, 等. 绵羊多胎主效基因FecB 分子检测方法的建立与应用[J]. 农业生物技术学报, 2009, 17(1):52-58.
[5] 王钧. 5个绵羊品种BMPR-IB基因多态性的研究[D]. 兰州:甘肃农业大学, 2013.
[6] 徐业芬. Smad信号转导通路相关基因表达与湖羊高繁殖力关系的研究[D]. 南京:南京农业大学, 2010.
[7] Ye L, Jiang WG.Bone morphogenetic proteins in tumour associated angiogenesis and implication in cancer therapies[J]. Cancer Letters, 2016, 380(2):586-597.
[8] Tseng JC, Chen HF, Wu KJ, et al.A twist tale of cancer metastasis and tumor angiogenesis[J]. Histology & Histopathology, 2015, 30(11):1283-1294.
[9] Maskur M, Tapaul R, Kasip L, et al.Genetic polymorphism of bone morphogenetic protein receptor 1B(BMPR1B)gene and its association with litter size in Indonesian fat-tailed sheep[J]. African Journal of Biotechnology, 2016, 15(25):1315-1319.
[10] Abdoli R, Zamani P, Deljou A, et al.Association of BMPR1B and GDF9 genes polymorphisms and secondary protein structure changes with reproduction traits in Mehraban ewes[J]. Gene, 2013, 524(2):296-303.
[11] 庞学燕, 季昀, 等. 奶牛αs-酪蛋白多克隆抗体的制备、纯化及鉴定[J]. 动物营养学报, 2012, 24(11):2190-2194.
[12] Careeira A, Alves GG, et al.Bone morphogenetic proteins:structure, biological function and therapeutic applications[J]. Arch Biochem Biophys, 2014, 561(6):64-73.
[13] Al-Samerria S.The role of bone morphogenetic proteins(BMPs)in ovarian function[D]. Australia:Curtin University, 2016.
[14] 贾建磊. 不同产羔性状绵羊卵巢组织差异表达蛋白质的筛选与分析[D]. 兰州:甘肃农业大学, 2015.
[15] Miao XY, Luo QM Qin XY. Genome-wide transcriptome analysis of mRNAs and microRNAs in Dorset and Small Tail Han sheep to explore the regulation of fecundity[J]. Molecular & Cellular Endocrinology, 2015, 402:32-42.
[16] 王钧, 张利平, 等. 5个绵羊品种BMPR-IB基因第7外显子多态性分析[J]. 中国农学通报, 2013, 29(29):46-51.
[17] Jia JL, Zhang LP, Wu JP, et al.Establishment of the optimum two-dimensional electrophoresis system of ovine ovarian tissue[J]. Genetics & Molecular Research, 2014, 13(3):6528-6538.
[18] Cao JY, Xu YP, Cai XZ, et al.TMT-based quantitative proteomics analyses reveal novel defense mechanisms of brassica napus, against the devastating necrotrophic pathogen sclerotinia sclerotiorum[J]. Journal of Proteomics, 2016, 143:265-277.
[19] Lu X, Hu B, Shao L, et al.Integrated analysis of transcriptomics and metabonomics profiles in aflatoxin B1-induced hepatotoxicity in rat[J]. Food Chem Toxicol, 2013, 55(3):444-455.
[20] Liu G, Zhang C, Wang G, et al.Molecular cloning, characterization and tissue specificity of the expression of the ovine CSRP2 and CSRP3 genes from Small tail Han sheep(Ovis aries)[J]. Gene, 2016, 580(1):47-57.
[21] Crispo M, Vilarino M, et al.Embryo development, fetal growth and postnatal phenotype of eGFP lambs generated by lentiviral transgenesis[J]. Transgenic Research, 2015, 24(1):31-41.
[22] Maskur R, Arman C.Association of a novel single nucleotide polymorphism in growth hormone receptor gene with production traits in Bali cattle[J]. Italian Journal of Animal Science, 2014, 13(4):3461-3466.
[23] Soukup V, Kozmik Z.The Bmp signaling pathway regulates development of left-right asymmetry in amphioxus[J]. Developmental Biology, 2017, 434(1):164-174.
[24] Antebi Y, Linton J, et al.Combinatorial signal perception in the BMP pathway[J]. Cell, 2017, 170(6):1184-1196.
[25] Teixeira F, Manfiolli A, Vieira N, et al.Fbxo25 regulates mapk signaling pathway through inhibition of erk1/2 phosphorylation[J]. Arch Biochem Biophys, 2017, 621(1):38-45.
[26] Ling J, Zhao K, Cui Y G, et al.Heat shock protein 10 regulated apoptosis of mouse ovarian granulosa cells[J]. Gynecological Endocrinology, 2011, 27(1):63-71.
[27] Wei LY, Zhang BW, et al.Exploring local discriminative informa-tion from evolutionary profiles for cytokine-receptor interaction prediction[J]. Neurocomputing, 2016, 217(12):37-45.
[28] Zhang TT, Zhang GM, Jin YH, et al.Energy restriction affect liver development in Hu sheep ram lambs through hippo signaling pathway[J]. Tissue & Cell, 2017, 49(5):603-611.
[29] 夏洪平, 杨惠玲. p38-MAPK信号转导通路及其与细胞凋亡的关系[J]. 癌变·畸变·突变, 2006, 3(2):255-256.
[30] Du M, Chen W, Zhang W, et al.TGF-β regulates the ERK/MAPK pathway independent of the SMAD pathway by repressing miRNA-124 to increase MALAT1 expression in nasopharyngeal carcinoma[J]. Biomed Pharmacother, 2018, 99:688-696.
[31] Xiao Y, Ye J, et al.Baicalin inhibits pressure overload-induced cardiac fibrosis through regulating AMPK/TGF-β/smads signaling pathway[J]. Arch Biochem Biophys, 2018, 640(15):37-46.
[32] Yang X, Huo H, Xiu C, et al.Inhibition of osteoblast differentiation by aluminum trichloride exposure is associated with inhibition of BMP-2/smad pathway component expression[J]. Food and Chemical Toxicology, 2016, 97:120-126.
[33] Chen Y, Nowak I, Huang J, et al.Erk/MAP kinase signaling pathway and neuroendocrine differentiation of non-small-cell lung cancer[J]. Journal of Thoracic Oncology, 2014, 9(1):50-58.
[34] Yosra B, Mongia A, Mar R, et al.Immune profiling of human prostate epithelial cells determined by expression of p38/TRAF-6/ERK MAP kinases pathways[J]. Kaohsiung Journal of Medical Sciences, 2017, 34(3):125-133.