Abstract: In order to better study and develop the pharmacological effect of the medicinal fungus Inonotus weigelae in the future,based on the tested indicators of mycelial biomass,pH,reducing sugar content,laccase activity,polyphenol content,MDA（Malonaldehyde）content,SOD（Superoxide dismutase）activity,T-AOC（Total antioxidant capacity）,and DPPH（1,1-diphenyl-2-picrylhydrazyl）radicals scavenging activity,the antioxidant activity of I. weigelae was evaluated during the liquid cultivation. The results demonstrated that the strain’s antioxidant activity was closely correlated with its mycelial growth,extracellular enzyme activity,and secondary metabolite secretion. The variations of mycelial biomass presented S-like curve,which increased rapidly from 6 to 12 d,reaching its peak at the 12th day. During the liquid cultivation,the increased peaks on reducing sugar content,laccase activity,and polyphenol content were also observed. Variation trends among the mycelial biomass,laccase activity,and polyphenol content were approximately consistent,indicating that there existed a mutual dependence between the nutrition utilization of this fungal strain and its physiological metabolism. The SOD activity and T-AOC during the culture tended to increase,indicating that the strain activated its antioxidant system to resist the damage from external environment under the oxidative stress. Additionally,I. weigelae exhibited remarkable scavenging capacity against DPPH radicals,showing the scavenging efficiency was up to 78.56% on the 2nd day and remaining over 50% on the 14th day. The above results confirmed that the medicinal fungus I. weigelae possesses the superior antioxidant activity.

Key words: medicinal fungus, Inonotus weigelae, mycelial biomass, laccase activity, antioxidant activity