Biotechnology Bulletin ›› 2016, Vol. 32 ›› Issue (11): 72-79.doi: 10.13560/j.cnki.biotech.bull.1985.2016.11.010

• Orginal Article • Previous Articles     Next Articles

Application Progress on Amplified Methods to Clone Flanking Sequence

PU Yuan-bo1, GUO Cui2, PING Shu-zhen2   

  1. 1. North Sichuan College of Preschool Teacher Education,Guangyuan 628017;
    2. Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081
  • Received:2016-07-13 Online:2016-11-25 Published:2016-11-11

Abstract: Amplifying the flanking sequence of genes plays an important role in molecular biology research. Until so far,the methods used to clone flanking sequence can been categorized into three groups:I-PCR,LA-PCR and TAIL-PCR. This paper briefly introduces the principle and present applications of these methods,which provides basis for researchers to choose more reliable and reasonable method.

Key words: flanking sequence, I-PCR, LA-PCR, TAIL-PCR