Research Progress on Optimizing the Expression of Exogenous Proteins in Escherichia coli

doi:10.13560/j.cnki.biotech.bull.1985.2017.02.003

Abstract

Abstract: Escherichia coli expression system has become the first choice of expression of exogenous protein,the expression system for recombinant protein presents many advantages,and however,sometimes expressed exogenous protein is easily attacked by host protease or cannot form insoluble inclusion bodies because of incorrect folding,thus by which the expression is limited. This paper summarizes some optimization strategies used in the process of the expression of exogenous proteins in E. coli,mainly including the expression of rare codons,application of fusion tags,change of E. coli strains for expression,and reducing the formation of inclusion bodies,a single protein production system,auto-induction,fed-batch cultivation,high cell-density culture,etc.,which aims at exploring the optimal strategy for the expression of exogenous protein.

Key words: Escherichia coli, exogenous protein, fed-batch cultivation, high-cell-density cultivation

SU Peng, GONG Guo-li. Research Progress on Optimizing the Expression of Exogenous Proteins in Escherichia coli[J]. Biotechnology Bulletin, 2017, 33(2): 16-23.

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