Patterns of Biofilm Formation in Pseudomonas stutzei Under Abiotic Stresses

doi:10.13560/j.cnki.biotech.bull.1985.2017.02.025

Abstract

Abstract: In order to systematically investigate the effects of abiotic stress factors on the growth and biofilm formation of Pseudomonas stutzeri A1501,we measured the variation patterns of growth and biofilm formation of A1501 under different NaCl concentrations,pH and temperature. The results revealed that above 3 environmental factors did influence the growth and the biofilm formation of A1501 strain with certain extent of regularity. The optimal growth condition of A1501 with those factors was 2 mmol/L NaCl,pH6.8,and 30℃,respectively. However,the biofilm formation reached the maximum in the stress condition of 0.6 mol/L NaCl,pH6.0,and 40℃ with 7.8-,7.0- or 2.0-fold increment compared to the optimal growth condition,respectively. Among the three factors,NaCl showed the most significant effect on the biofilm formation of A1501. In a conclusion,under the tested conditions,the biofilm formation of A1501 strain was negatively correlated with the growth of strain,i.e.,high salt,weak acid and high temperature were adverse to the growth of A1501,however,it stimulated the biofilm formation.

Key words: Pseudomonas stutzei A1501, abiotic stress, biofilm formation, growth characteristics, environmental factor

YAN Ning, YANG Zhi-min, SHANG Li-guo, DAI Shu-ling, ZHAN Yu-hua, LU Wei, LIN Min, YAN Yong-liang. Patterns of Biofilm Formation in Pseudomonas stutzei Under Abiotic Stresses[J]. Biotechnology Bulletin, 2017, 33(2): 172-178.

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