Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (2): 164-171.doi: 10.13560/j.cnki.biotech.bull.1985.2017.02.024

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Effect of Ferritin DrfE on Antioxidant Enzyme Activity in Deinococcus radiodurans

WU Xiao-li1,2, LIU Ying-ying2, JIANG Shi-jie2,3, CHEN Yun2, LIU Xiao-li1,2, WANG Yu-zhou4, PING Shu-zhen2, WANG Jin1,2   

  1. 1. College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621000;
    2. Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081;
    3. College of Life Sciences,Sichuan University,Chengdu 610065;
    4. The High School Affiliated to Renmin University of China,Beijing 100080
  • Received:2016-11-07 Online:2017-02-26 Published:2017-02-08

Abstract: To investigate the function of the ferritin DrfE encoded by gene drfE in Deinococcus radiodurans,we constructed the complemented strain pdrfE∷Δ,and compared the stress resistances of the wild-type WT,mutant ΔdrfE and complemented strain pdrfE∷Δ under different doses of hydrogen peroxide(H2O2)and NaCl. Also,the Fe2+concentration and antioxidant enzyme activities of three strains were measured under oxidative stress. The results showed that the deletion of gene drfE led the strain to be more sensitive to oxidative and high-salt stresses and the complementation of gene drfE restored the resistance of the strain to oxidative and salt stress. Furthermore,the deletion of drfEresulted in the Fe2+ concentration in D. radiodurans cell increased from 232 μmol/L to 293 μmol/L in vivo. After treated with 80 mmol/L H2O2 for 30 min,the CAT and SOD activities of the mutant ΔdrfE decreased by 32.74% and 41.3%,respectively,than that of wild-type. The above findings implied that the ferritin DrfE might be involved in iron metabolic pathway of D. radiodurans and played a key role in antioxidant system.

Key words: Deinococcus radiodurans, ferritin DrfE, abiotic stress, antioxidant enzyme, iron ion