Comparative Analysis of Identification Methods of CP4-EPSPS Transgenic Cotton Plants

doi:10.13560/j.cnki.biotech.bull.1985.2017-0250

Abstract

Abstract: CP4-EPSPS isolated from Agrobacterium strain CP4 is a glyphosate resistant gene and one of the most commonly used marker genes in transgenic plant research. However,we found that investigated results of the transgenic plants were not always completely accurate only using simple glyphosate resistance assay in the identification of CP4-EPSPS transgenic plants,which resulted in false-positive plants or missing positive transgenic plants. In this study,by use CP4-EPSPS transgenic cottons that we had gained,the four screening methods of CP4-EPSPS transgenic plants were compared,which included PCR,ELISA,resistant test strips and glyphosate resistance assay. The results show that the accuracy of ELISA and test strip are significantly higher than PCR and 0.3% glyphosate,but the test strip test is more convenient than ELISA basing on the comprehensive analysis,accuracy,timeliness,ease of use and price in identification of transgenic plants. Therefore we think combination glyphosate resistance assay with resistant strip test is the best choice. Our finding provides significant guidance for people engaging in the identification of CP4-EPSPS transgenic plants both in the laboratory and field.

Key words: transgenic cotton, CP4-EPSPS, glyphosate resistance, identification of transgenic plants

GUO Wen-fang, WANG Nan, LI Gang-qiang, XU Fang-fang, YANG Cai-feng, LIU De-hu. Comparative Analysis of Identification Methods of CP4-EPSPS Transgenic Cotton Plants[J]. Biotechnology Bulletin, 2017, 33(4): 114-118.

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