Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (5): 159-163.doi: 10.13560/j.cnki.biotech.bull.1985.2016-0953

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Effects of Polysaccharides from Lu Dangshen(Codonopsis pilosula)on Proliferation and Migration of Human Cervical SiHa

HU Jian-ran LI Ping LEI Hai-ying LIU Xian-rui   

  1. Department of Biological Sciences and Technology of Changzhi University,Changzhi 046011
  • Received:2016-10-19 Online:2017-05-25 Published:2017-05-19

Abstract: The effects of polysaccharides from Lu Dangshen(Codonopsis pilosula)(LDP)on the proliferation and migration of human cervical cancer cell SiHa was investigated. MTT method was used to determine the effects of LDP on SiHa cell proliferation,and cell adhesion assay,cell spreading assay and cell wound scratch assay were used to analyze the effect of LDP on SiHa cell migration. LDP significantly repressed SiHa cell proliferation(IC50 = 0.68 mg/mL),and inhibited adhesion between cells and extracellular matrix,and delayed cell spreading,and then restrained SiHa cell migration. LDP presents solid inhibition effects on the proliferation,adhesion,spreading and migration of human cervical cancer cell SiHa,and thus possesses potential value for developing auxiliary medicine for tumor treatment and dietary supplement.

Key words: polysaccharides from Lu Dangshen(Codonopsis pilosula), cell proliferation, cell adhesion, cell spreading, cell migration