Effects of Total Flavonoids from Forsythia suspense on the Proliferation of Gastric Cancer Cell MGC80-3

doi:10.13560/j.cnki.biotech.bull.1985.2017-1054

Abstract

Abstract: Effects of Changzhi Forsythia suspense flavonoids（FF）on the proliferation of gastric cancer cell MGC80-3 were investigated. Total flavonoids were extracted from Changzhi F. suspense using the ethanol reflux method. MTT method，colony formation assay，RT-PCR，and Western blot were used to access the inhibitory effects of FF on MGC80-3 cell proliferation and the potential molecular mechanism. The results showed that FF significantly inhibited the proliferation and colony formation of MGC80-3 cells，and its molecular mechanism was that FF down-regulated the protein level of mTOR and up-regulated the expressions of Bax as well as cellular autophagy factor Beclin 1 and LC3 II，which promoted the autophagic cell death，then inhibiting the survival of MGC80-3 cells. Therefore, FF possesses the potential value for developing anti-tumor drug.

Key words: Changzhi Forsythia suspense, flavonoids, cell proliferation, autophagic cell death

LI Ping ,ZHANG Gui-ping, HU Jian-ran. Effects of Total Flavonoids from Forsythia suspense on the Proliferation of Gastric Cancer Cell MGC80-3[J]. Biotechnology Bulletin, 2018, 34(6): 199-203.

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