Screening，Identification of Multifunctional Peanut Root-promoting Rhizobacteria and Its Promoting Effects on Peanuts（Arachis hypogaea L.）

doi:10.13560/j.cnki.biotech.bull.1985.2017-0233

Abstract

Abstract: This study aims to acquire the peanut root-promoting rhizobacteria（PGPR）with multifunction of fixing nitrogen，dissolving phosphorus and potassium，and synthesizing the auxin，for increasing and improving the yield and quality of peanuts in Henan. Five PGPR strains of HS4，HS7，HS9，HS10，and HS11 were screened from sandy alluvial soil of peanut rhizosphere in North China. Further HS9 was chosen by comparing various functional indexes. The activity of nitrogenase，dissolving phosphorus and potassium and IAA synthesis of HS9 were 15.53 nmol C₂H₄/h·mL，279.23 mg/L，and 22.5 mg/L and 40.96 mg/L， respectively. HS9 was identified as Bacillus flexus based on the morphological observation，16S rDNA gene sequence analysis accompanied with physiological and biochemical test. Pot experiment was then conducted to test and verify the effects of growth promoting，and the results showed that inoculation with HS9 significantly increased the content of available phosphorus and potassium in soil，and distinctly enhanced the content of IAA in soil. This led to the root length，surface area，root volumes and root tips of peanut increased 109.60%，84.30%，76.08% and 386.24%，respectively. It indicated that the strain promoted plant nutrient uptake and utilization，and increased plant biomass and nutrient content. The average wet weight of plants was 1.7 times higher than the control treatment and the height of peanut inoculated with HS9 significantly increased by 28.35%；the SPAD（Soil and plant analyzer Development）value significantly increased by 16.06% and the total N，P and K content of peanut significantly increased by 23.11%、83.04% and 23.95%，respectively. Conclusively，the multifunctional strain HS9 has solid growth-promoting effect on peanut and broad agricultural application prospect.

Key words: peanuts, nitrogen fixing, dissolving phosphorus and potassium, indole-3-acetic acid, root-promoting rhizobacteria

LIU Ye, LIU Xiao-dan, ZHANG Lin-li, WU Yue, WANG Guo-wen, WANG iang, JIANG Ying. Screening，Identification of Multifunctional Peanut Root-promoting Rhizobacteria and Its Promoting Effects on Peanuts（Arachis hypogaea L.）[J]. Biotechnology Bulletin, 2017, 33(10): 125-134.

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