Application Analysis of Microcystis aeruginosa Identification Based on Ribosomal Proteins

doi:10.13560/j.cnki.biotech.bull.1985.2017-0268

Abstract

Abstract: This research is to verify the feasibility of matrix-assisted laser desorption-ionization-time-of -flight mass spectrometry（MALDI-TOF MS）in the identification of mixed and actual cyanobacterial samples by employing ribosomal proteins as biomarkers. Mechanical disruption and high speed centrifugation were used as pre-treatments to collect the ribosomal protein fraction of cyanobacterial samples，and then MALDI-TOF MS was applied to identify Microcystis aeruginosa in samples after proteins in situ crystallization with proteomic analysis based on 13 ribosomal proteins as biomarkers. The results showed that under the optimal conditions，model strain M. aeruginosa was precisely identified in the mixed samples and the detection rates of assigned peaks were all greater than 75% with reasonable errors range except the fourth sample. The lowest biomass detection limit and ratio of M. aeruginosa was 2.88×10^{6 cells and 37%，respectively. Furthermore，M. aeruginosa was identified and detected in the cyanobacterial samples from Taihu Lake and the detection rate of assigned peaks was 76.9%. In conclusion，MALDI-TOF MS employing ribosomal proteins as biomarkers could be successfully applied to the identification of mixed and actual cyanobacterial samples and will be expected as routine tool in environmental cyanobacteria monitoring.}

Key words: ribosomal protein, Microcystis aeruginosa, MALDI-TOF MS, cyanobacterial bloom

JIANG Wen-jing, ZHANG Jun-yi, DU Yang, SUN Li-wei. Application Analysis of Microcystis aeruginosa Identification Based on Ribosomal Proteins[J]. Biotechnology Bulletin, 2017, 33(10): 117-124.

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