The Screening of β-Glycosidase-producing Strain and the Transforming of Resveratrol

doi:10.13560/j.cnki.biotech.bull.1985.2017-0439

Abstract

Abstract: Resveratrol has a lot of benefits such as anti-cancer and anti-oxidation,and thus is widely applied in cosmetics,medicine and so on. This study aims to screen bacteria which can secrete β-glycosidase and study it’s ability of transforming polydatin. Through the geniposide plate screening and polydatin shake flask,a strain secreting β-glucosidase and transforming polydatin to resveratrol was acquired. It was identified as Bacillus safensis by 16S rDNA sequence analysis,named as CGMCC 13129. Under these conditions of 37℃,with 7 percent of inoculation,pH7,and conversing for 8 h,the conversion rate of polydatin was up to 90%. HPLC,HPLC-MS and ¹H-NMR were used to test and identify the product as resveratrol,and the purity of the product was up to 99.3% by one-time methanol extraction.

Key words: resveratrol, Bacillus safensis, β-glycosidase enzyme, polydatin

FENG Wei, HU Xiao-yan, MA Ming-na, GUO Meng, LU Fu-ping, LI Yu. The Screening of β-Glycosidase-producing Strain and the Transforming of Resveratrol[J]. Biotechnology Bulletin, 2017, 33(11): 130-135.

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