Cloning and Identification of Gene Promoter for Gliotoxin Biosynthesis from Deep-Sea-Derived Fungus Dichotomomyces cejpii

doi:10.13560/j.cnki.biotech.bull.1985.2017-0941

Abstract

Abstract: The promoters of gliotoxins biosynthesis genes including GliG,GliI and GliO from deep-sea-derived fungus Dichotomomyces cejpii were cloned by the method of genome walking,and the core regions of these promoters were inserted into the reporter gene vector of luciferase pGL-3-basic,and the transcriptional activities of GliG promoter was the highest according to its fluorescence intensity. Further,the core region of GliG promoter was further inserted into the pAN7-1 vector with hygromycin resistance marker to replace original pgpdA promoter,then the recombinant plasmid was transferred into Saccharomyces cerevisiae by electronic transformation. The positive clones were screened by plate with hygromycin resistance,and the results demonstrated that the GliG promoter initiated the expression of hygromycin resistance gene.

Key words: Dichotomomyces cejpii, gliotoxin, promoter, cloning, identification, marine fungus

HUANG Zi-lei, ZHANG Wei-min, YE Wei, LI Sai-ni, LI Hao-hua, ZHU Mu-zi. Cloning and Identification of Gene Promoter for Gliotoxin Biosynthesis from Deep-Sea-Derived Fungus Dichotomomyces cejpii[J]. Biotechnology Bulletin, 2018, 34(4): 144-150.

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