Biotechnology Bulletin ›› 2018, Vol. 34 ›› Issue (5): 32-40.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0877

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CRISPR/Cas9 Genome Editing Technology and Its Application in Streptomyces

QIAO Long-liang, PANG Jian-hu, DANG Chen-yang, HUANG Hai-long, ZHU Peng   

  1. School of Marine Sciences,Ningbo University,Ningbo 315211
  • Received:2017-10-19 Online:2018-05-26 Published:2018-06-07

Abstract: Streptomyces are known to produce a variety of antibiotics and have long been a research focus in life sciences and medicine,pharmacy,and industry. However,the genetic manipulation of Streptomyces is very difficult,thus the traditional means of genetic editing due to low efficiency,long experimental cycle and cumbersome steps and other reasons are gradually replaced by CRISPR/Cas9 system called as “magic scissors”. This technique allows for targeted editing of genome-specific sites,including indel,repair and replacement. By comparing the current gene editing methods in Streptomyces,it is concluded that CRISPR/Cas9 system has the advantages of simple operation,high efficiency,low cost,short experimental cycle and the ability to simultaneously knock out multiple genes in the application of Streptomyces. We also review the application of the system in Streptomyces and summarize the target space of CRISPR/Cas9 toolbox. Finally,we prospect the potential of CRISPR/Cas9 system in Streptomyces orientated-breeding and synthetizing new antibiotics,aiming at providing references for the work of related fields.

Key words: CRISPR/Cas9, genome editing, specificity, high efficiency, Streptomyces