Abstract: The effects of methyl jasmonate（MJ）on the content of secondary metabolites in Salvia miltiorrhiza hairy roots were studied to provide experimental basis for the production and application of culturing hairy roots of S. miltiorrhiza. Having methyl jasmonate as the inducer,the hairy root culture of S. miltiorrhiza was induced. The results showed that the contents of tanshinone Ⅰ and tanshinone ⅡA of S. miltiorrhiza hairy roots with MJ were 1.53 times and 1.16 times higher than those of hairy roots without MJ respectively. Similarly,the contents of rosmarinic acid and salvianolic acid B were 1.37-fold and 4.43-fold increase in S. miltiorrhiza hairy roots with MJ. The results confirmed that MJ was an efficient inducer,which significantly improved the synthesis and accumulation of the secondary metabolites in S. miltiorrhiza hairy roots. In another words,induction by MJ could be used for the efficient production of effective secondary metabolites of S. miltiorrhiza.

Key words: methyl jasmonate, Salvia miltiorrhiza, hairy root, active ingredient content