Biotechnology Bulletin ›› 2018, Vol. 34 ›› Issue (3): 87-92.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0780

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Effects of Exogenous Methyl Jasmonate on the Tolerance of Wheat Seedlings to Low Temperature

LI Yang-yang, JIAO Zhen   

  1. School of Physical Engineering,Zhengzhou University,Zhengzhou 450052
  • Received:2017-09-18 Online:2018-03-20 Published:2018-04-10

Abstract: The effects of exogenous methyl jasmonate on the physiological and biochemical characteristics and the expression of cold-resistant transcription factor in wheat seedlings under cold acclimation were studied with cultivar “Aikang 58”. The test wheat seedlings were set in two groups:the controlled group was sprayed with double distilled water at low temperature of 4℃,and the treated group was sprayed with 100 μmol/L of methyl jasmonate at 4℃ for a week. The physiological and biochemical results showed that the survival rate of wheat seedling treated with 100 μmol/L of exogenous methyl jasmonate was 91%,significantly increased than that of the controlled group. The SOD、CAT and POD activity of the treated group increased 34%、 50% and 14% than that of the controlled group,respectively,the content of soluble proteins increased 30%,and intracellular free proline content increased 14%,while MDA content decreased by 5%. There was a significant difference between the treated group and the controlled group. The quantitative real-time PCR showed that 100 μmol/L of exogenous methyl jasmonate significantly increased expression of cold responsive transcription factors TaCBFⅣd-B4,TaCBFⅢd-B19,TaCBFⅢc-D3,and TaCBFⅢd-D19,and the expression of TaCBFⅣd-B4 was the highest. The results indicated that spraying 100 μmol/L exogenous methyl jasmonate enhanced the tolerance of wheat seedlings to low temperature stress.

Key words: exogenous methyl jasmonate, wheat, low temperature tolerance, physiological and biochemical characteristics, cold-resistant transcription factor TaCBFs