Preliminary Study on Preservative Efficacy and Safety Evaluation of Antimicrobial Peptides in Shampoo

doi:10.13560/j.cnki.biotech.bull.1985.2018-0288

Abstract

Abstract: Preservatives are important ingredients to prevent cosmetics from microbial contamination. In order to have effective but non-toxic preservatives,the MICs of four antimicrobial peptides were determined,and two peptides N2 and NZ2114 having favorable antibacterial effects towards Gram-negative and -positive bacteria were screened. The preservative effect and safety performance were measured and evaluated by antibacterial experiments in vitro,ROS level and skin irritation test in mice. The results showed that,N2 and NZ2114 had a fast and efficient sterilization effect at the low concentration scope（40 and 20 µg/mL respectively）,and the cell survival rate was more than 85% at a high concentration（128 µg/mL）,moreover,they had low cytotoxicity. The results of ROS level and skin irritation test in mice demonstrated that N2 and NZ2114 did not damage skin cells and had antioxidant effect to some extent. These results provide a reference for using antimicrobial peptides a potent candidate of new effective and safe preservative in shampoo.

Key words: preservative, antibacterial peptides, preservative effect, safety performance

GUI Yin-xue, YANG Na, TENG Da, WANG Xiu-min, MAO Ruo-yu, HAO Ya, TENG Guo-xin, WANG Jian-hua. Preliminary Study on Preservative Efficacy and Safety Evaluation of Antimicrobial Peptides in Shampoo[J]. Biotechnology Bulletin, 2018, 34(10): 217-224.

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