Proteomic Analysis of Desert Chlorella Under Drought Stress

doi:10.13560/j.cnki.biotech.bull.1985.2018-0423

Abstract

Abstract: By studying the proteomic changes of Chlorella under drought stress,the response mechanism of Chlorella to drought stress was explained from the level of protein expression. The total proteins of Chlorella treated with 20% PEG 6000 for 0,6,12,18,24,and 30 d were extracted. Two-dimensional electrophoresis and mass spectrometry techniques were applied to analyze the differential proteins. Totally 29 differential proteins were identified and classified into 7 categories based on function：photosynthesis,energy synthesis and transformation,substance metabolism,antioxidation,transport and cell structure,stress resistance,and unknown function proteins. The changes in the expressions of these proteins affect the accumulation of oil in the desert Chlorella,play a crucial role in the response to drought stress,and directly or indirectly are involved in the photosynthesis and oil synthesis in the cells of desert Chlorella.

Key words: desert Chlorella, drought stress, proteome, differentially expressed proteins

TAN Jun, MOU Yun, ZHOU Guang-pu, ZHOU Yong-shun, XU Jie, GAO Jian-feng. Proteomic Analysis of Desert Chlorella Under Drought Stress[J]. Biotechnology Bulletin, 2018, 34(10): 207-216.

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