Effects of Exogenous Paclobutrazol and GA3 Treatment on the Accumulation of Endogenous IAA and Transcriptional Level of Related Genes in the Development of Tuberous Toot of Pseudostellaria heterophylla

doi:10.13560/j.cnki.biotech.bull.1985.2018-0754

Abstract

Abstract: ELISA and Real-iime quantitative PCR experiments were conducted for investigating the dynamics of endogenous IAA and transcriptional level of related genes in response to exogenous paclobutrazol and GA3 treatment on P. heterophylla. The results showed that after PBZ treatment,the content of endogenous IAA by PBZ treatment at 10 d,20 d and 30 d was higher than the control groups after the first treatment,while lower than the control groups after 30 d. The content of endogenous IAA in the tuberous root by GA3 treatment showed fluctuation,i.e.,it was always higher than the control groups in other periods except for the 20d after treatment. In addition,the expression levels of genes related to endogenous IAA biosynthesis were analyzed in tuberous roots of P. heterophylla treated at 50 d after exogenous PBZ and GA3 treatment. The results showed that the Unigene37777 was up-regulated by PBZ treatment,while the expression of Unigene43146 and Unigene43412 were inhibited and the expression was down-regulated,but the three genes were induced by exogenous GA3;however,Unigene49937 presented opposite expression pattern in the treatment of exogenous PBZ and GA3,it was induced by PBZ,but inhibited by GA3. Overall,PBZ and GA3 induced the accumulation of endogenous IAA during the development of P. heterophylla,and had varied responses to the expressions of synthesis related-genes.

Key words: Pseudostellaria heterophylla, GA3, paclobutrazol, IAA

ZHENG Wei, ZHOU Tao, JIANG Wei-ke, LI Jun, XIAO Cheng-hong, YANG Chang-gui, ZHANG Chen, GONG An-hui, WEI De-qun, BI Yan. Effects of Exogenous Paclobutrazol and GA3 Treatment on the Accumulation of Endogenous IAA and Transcriptional Level of Related Genes in the Development of Tuberous Toot of Pseudostellaria heterophylla[J]. Biotechnology Bulletin, 2019, 35(2): 46-52.

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