Screening and Identification of Phosphate Solubilizing Bacteria from Maize Rhizosphere Soil and Its Growth Promoting Effect

doi:10.13560/j.cnki.biotech.bull.1985.2019-0678

Abstract

Abstract:

In order to obtain plant growth promoting rhizobacteria（PGPR）in maize and clarify its growth-promoting characteristics,PGPR strain were screened from maize rhizosphere soil samples. Then the P-solubilizing characteristics and IAA-producing ability of preferred strains were studied using soil pot experiment. The strains were further identified and their growth-promoting effects were investigated in pot experiment. As results,2 excellent PGPR strains were isolated and named as CH07 and FD11,and their solubilized phosphorus reached 368.5 mg/L and 321.5 mg/L,the produced IAA（indole acetic acid）were 30.93 mg/L and 15.93 mg/L,respectively. According to the results of morphologic characteristics,physiological biochemical properties and phylogenetic analysis of 16S rRNA genes,CH07 was identified as Bacillus aryabhattai and FD11 as Streptomyces maritimus. Finally,the growth promoting effects of the two bacterial isolates were compared by pot experiment. The results showed that CH07,FD11,especially the mixture of CH07 and FD11,had a positive effect on the plant height and shoot fresh weight of amaranth,and could be used as an excellent strain resource for the development of biological fertilizer.

Key words: phosphate solubilizing, indole acetic acid（IAA）, plant growth-promoting rhizobacteria, screening and identification

WAN Shui-xia, WANG Jing, LI Fan, JIANG Guang-yue, XU Wen-jing, LIU Zuo-jun. Screening and Identification of Phosphate Solubilizing Bacteria from Maize Rhizosphere Soil and Its Growth Promoting Effect[J]. Biotechnology Bulletin, 2020, 36(5): 98-103.

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