Isolation and Identification of Flavonoids-producing Endophytic Fungi in Papaya

doi:10.13560/j.cnki.biotech.bull.1985.2018-0857

Abstract

Abstract: In order to develop the microbial resources of endophytic fungi,endophytic fungi were isolated from the leaves,fruits and stems of papaya plants by tissue separation. The secondary metabolites of endophytic fungi from papaya were preliminarily analyzed by color reaction of flavonoids,thin layer chromatography,and UV spectroscopy. One strain of endophytic fungus producing flavonoids was screened,and the strain was analyzed and identified by using the conidial morphological characteristics and the internal transcribed spacer. The results showed that the endophytic fungus strain G41 isolated from papaya fruit could be classified as Penicillium citrinum. This study confirms that there are endophytic fungi strains producing flavonoids in papaya fruit,which demonstrates potential value for further development and utilization.

Key words: plant endophytic fungi, flavone, Penicillium citrinum

KE Shu-wei, LÜ Jin-hui, CHEN Ping, ZHANG Rong-ping, YU Yue. Isolation and Identification of Flavonoids-producing Endophytic Fungi in Papaya[J]. Biotechnology Bulletin, 2019, 35(3): 47-52.

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