Isolation of Endophytic Fungi from Cinnamomum camphora Leaves,Screening and Identification of Biologically Active Strains

doi:10.13560/j.cnki.biotech.bull.1985.2018-0750

Abstract

Abstract: This work aims to isolate endophytic fungi from leaves of Cinnamomum camphora,to determine its anti-bacterial activity,anti-tumor activity and anti-oxidation activity in vitro,and to screen and identify strains with strong biological activity. On the isolated endophytic fungi,K-b disk method was used to determine its anti-bacterial activity,MTT assay to determine its anti-tumor activity,ultraviolet spectrophotometry to determine its anti-oxidation activity,and ITS（Internal transcribed spacer）to analyze and determine the species of active strains. Results showed that total 39 endophytic fungi were isolated from the leaves of C. camphora.Among them,there were 7 strains highly inhibiting Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Pseudomonas aeruginosa. Further,the strain ZS14 with anti-tumor and anti-oxidation activities from the 7 strains was screened,its inhibition rate on human breast cancer cell MCF-7 reached 73.00%,clearance rate of DPPH was 70.92%,scavenging rate of hydroxyl radical was 78.83%,and clearance rate of hydrogen peroxide was 81.36%. With ITS sequencing results it was designated as Diaporthales sp. ZS14. In sum,the isolated strain Diaporthales sp. ZS14 had strong anti-bacterial,anti-tumor and anti-oxidation activities,and it can be used as potential sources for drug development in further study.

Key words: endophytic fungi, anti-bacterial, anti-tumor, anti-oxidation, isolation and identification

JIN Hong-jie, CAO Hong, LIU Hong, ZHENG Shuang, JIANG Chao. Isolation of Endophytic Fungi from Cinnamomum camphora Leaves,Screening and Identification of Biologically Active Strains[J]. Biotechnology Bulletin, 2019, 35(3): 53-58.

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