Screening and Degradation Characteristics of a Strain Degrading Polyacrylamide

doi:10.13560/j.cnki.biotech.bull.1985.2019-0292

Abstract

Abstract: A bacterial strain degrading polyacrylamide was screened from the sludge containing polyacrylamide（PAM）. The bacterium can grow with polyacrylamide as the sole carbon source and nitrogen source. The degradation rate of PAM by the strain（inoculation 5%）reached 43% within 48 h when the initial concentration of PAM was 1.2 g/L,the temperature was 35℃,and the pH was 7.0. The strain was identified as Klebsiella sp.,named PCX,after physiological,biochemical and 16S rDNA sequence analysis. The molecular weight of the degraded product of PAM（molecular weight：3×10³ kD）was 0.619 kD. Combined with the results from infrared spectroscopy,the amide group on the molecular chain was hydrolyzed to carboxyl group and the side chain was degraded. The results of acute biological toxicity analysis showed that the EC₅₀ was 106 mg/L,indicating that the degraded products had no toxic effect on aquatic organisms.

Key words: strain identification, Klebsiella, polyacrylamide, degradation characteristics

LIU Yu-cheng, QIU Lian, LIANG Jing-jing, LI Ling-li, MA Li-li. Screening and Degradation Characteristics of a Strain Degrading Polyacrylamide[J]. Biotechnology Bulletin, 2019, 35(9): 178-183.

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