Cloning, Expression Analyses of HbAIH Gene from Hevea brasiliensis

doi:10.13560/j.cnki.biotech.bull.1985.2019-0940

Abstract

Abstract: Agmatine iminohydrolase（AIH）in plant is one of the most important enzymes in the arginine decarboxylase pathway in which arginine is subsequently converted to poluamine. This study is the first time for an AIH gene，HbAIH，to be cloned from Hevea brasiliensis. The full-length sequence of HbAIH was 1 462 bp with a 1 137 bp open reading frame，encoding a sequence with 378 amino acid residues. HbAIH was a hydrophilic protein，with a calculated molecular weight of 42.28 kD and an isoelectric point of 5.09. Sequence alignment revealed that the deduced amino acid sequence of HbAIH presented the high similarity with those of MeAIH in Manihot esculenta，RcAIH in Ricinus communis and JcAIH in Jatropha curcas from Euphorbiaceae，and also contained 11 highly conservative amino acid residues that formed the active sites and participated in the binding with the substrate. qRT-PCR analyses demonstrated that HbAIH expressed with no tissue specificity，the highest in female flowers，followed by barks，stem apexes，latex，leaves and male flowers. HbAIH expression significantly varied in different developmental stages of rubber tree leaves，with the highest expression in light young stage and the lowest one in mature stage，suggesting that HbAIH might be associated with leaf development. In addition，the expression of HbAIH was induced by wounding，low temperature，drought，high salt，hydrogen peroxide，ethrel，MeJA treatments，and tapping panel dryness. All these results suggest that HbAIH is likely involved in growth and development of H. brasiliensis，regulating latex biosynthesis and latex flow，as well as stress responses.

Key words: Hevea brasiliensis, agmatine iminohydrolase, stress responses, expression analysis

YANG Hong, YUE Yi-fan, HU Yan-ling, DENG Zhi, DAI Long-jun, LI De-jun. Cloning, Expression Analyses of HbAIH Gene from Hevea brasiliensis[J]. Biotechnology Bulletin, 2020, 36(5): 120-129.

References

[1] Takano A, Kakehi JI, Takahashi T.Thermospermine is not a minor polyamine in the plant kingdom[J]. Plant Cell Physiol, 2012, 53
(4):606-616.
[2] Jiménez-Bremont JF, Marina M, Guerrero-González ML, et al.Physiological and molecular implications of plant polyamine metabolism during biotic interactions[J]. Front Plant Sci, 2014, 5:95.
[3] Minocha R, Majumdar R, Minocha SC.Polyamines and abiotic stress in plants:a complex relationship[J]. Front Plant Sci, 2014, 5:175.
[4] Tiburcio AF, Altabella T, Bitrián M, et al.The roles of polyamines during the lifespan of plants:from development to stress[J]. Planta, 2014, 240(1):1-18.
[5] Liu JL, Wang WX, Wang LY, et al.Exogenous melatonin improves seedling health index and drought tolerance in tomato[J]. Plant Growth Regul, 2015, 77(3):317-326.
[6] Hussain SS, Ali M, Ahmad M, et al.Polyamines:Natural and engineered abiotic and biotic stress tolerance in plants[J]. Biotechnol Adv, 2011, 29(3):300-311.
[7] Gill SS, Tuteja N.Polyamines and abiotic stress tolerance in plants[J]. Plant Signal Behav, 2010, 5(1):26-33.
[8] Igarashi K, Kashiwagi K.Modulation of cellular function by polyamines[J]. Int J Biochem Cell Biol, 2010, 42(1):39-51.
[9] Pottosin I, Velarde-Buendía AM, Bose J, et al.Cross-talk between reactive oxygen species and polyamines in regulation of ion transport across the plasma membrane:implications for plant adaptive responses[J]. J Exp Bot, 2014, 65(5):1271-1283.
[10] Pottosin I, Shabala S.Polyamines control of cation transport across plant membranes:implications for ion homeostasis and abiotic stress signaling[J]. Front Plant Sci, 2014, 5:154.
[11] Radhakrishnan R, Lee IJ.Spermine promotes acclimation to osmotic stress by modifying antioxidant, abscisic acid, and jasmonic acid signals in soybean[J]. Plant Growth Regul, 2013, 32(1):22-30.
[12] Kamiab F, Talaie A, Khezri M, et al.Exogenous application of free polyamines enhance salt tolerance of pistachio(Pistacia vera L.)seedlings[J]. Plant Growth Regul, 2014, 72(3):257-268.
[13] Mostofa MG, Yoshida N, Fujita M.Spermidine pretreatment enhances heat tolerance in rice seedlings through modulating antioxidative and glyoxalase systems[J]. Plant Growth Regul, 2014, 73(1):31-44.
[14] Martin-Tanguy J.Metabolism and function of polyamines in plants:recent development(new approaches)[J]. Plant Growth Regul, 2001, 34(1):135-148.
[15] Wimalasekera R, Tebartz F, Scherer GFE.Polyamines, polyamine oxidases and nitric oxide in development, abiotic and biotic stresses[J]. Plant Sci, 2011, 181(5):593-603.
[16] Subramanyam S, Sardesai N, Minocha SC, et al.Hessian fly larval feeding triggers enhanced polyamine levels in susceptible but not resistant wheat[J]. BMC Plant Biology, 2015, 15(1):3.
[17] Michael AJ.Evolution of biosynthetic diversity[J]. Biochem J, 2017, 474(14):2277-2299.
[18] Hanfrey C, Sommer S, Mayer MJ, et al.Arabidopsis polyamine biosynthesis:absence of ornithine decarboxylase and the mechanism of arginine decarboxylase activity[J]. Plant J, 2001, 27(6):551-560.
[19] Yanagisawa H, Suzuki Y.Corn agmatine iminohydrolase:purification and properties[J]. Plant Physiol, 1981, 67(4):697-700.
[20] Yanagisawa H.Agmatine deiminase from maize shoots:purification and properties[J]. Phytochemistry, 2001, 56(7):643-647.
[21] Park KH, Cho YD.Purification of monomeric agmatine iminohydrolase from soybean[J]. Biochem Biophys Res Commun, 1991, 174(1):32-36.
[22] Janowitz T, Kneifel H, Piotrowski M.Identification and characterization of plant agmatine iminohydrolase, the last missing link in polyamine biosynthesis of plants[J]. FEBS Lett, 2003, 544(1-3):258-261.
[23] 黄雪梅, 杨少琼, 黎瑜, 等. 多胺在RRIM600 橡胶树割面上的分布及与割胶的关系[J]. 热带作物学报, 2000, 21(4):15-19.
[24] Zhao M, Liu H, Deng Z, et al.Molecular cloning and characterization of, adenosylmethionine decarboxylase gene in rubber tree[J]. Physiology and Molecular Biology of Plants, 2017, 23(2):281.
[25] 侯岚菲, 杨洪, 邓治, 等. 橡胶树ADC1的克隆、表达及生物信息学分析[J]. 生物技术通报, 2018, 34(11):117-125.
[26] 刘辉, 邓治, 陈江淑, 等. 巴西橡胶树类钙调素蛋白基因 HbCML27 克隆与表达分析[J]. 分子植物育种, 2015, 13(12):2721-2727.
[27] Tang CR, Huang DB, Yang JH, et al.The sucrose transporter HbSUT3 plays an active role in sucrose loading to laticifer and rubber productivity in exploited trees of Hevea brasiliensis(para rubber tree)[J]. Plant Cell Environ, 2010, 33(10):1708-1720.
[28] Zhu JH, Zhang QQ, Wu R, et al.HbMT2, an ethephon-induced metallothionein gene from Hevea brasiliensis responds to H₂O₂ stress[J]. Plant Physiol Biochem, 2010, 48(8):710-715.
[29] Hao BZ, Wu JL.Laticifer differentiation in Hevea brasiliensis:induction by exogenous jasmonic acid and linolenic acid[J]. Annals of Botany, 2000, 85(1):37-43.
[30] Robert X, Gouet P.Deciphering key features in protein structures with the new ENDscript server[J]. Nucleic Acids Res, 2014, 42(W1):W320-W324.
[31] Hartzoulakis B, Rossiter S, Gill H, et al.Discovery of inhibitors of the pentein superfamily protein dimethylarginine dimethylaminohydrolase(DDAH), by virtual screening and hit analysis[J]. Bioorg Med Chem Lett, 2007, 17(14):3953-3956.
[32] Sekula B, Dauter Z.Structural study of agmatine iminohydrolase from Medicago truncatula, the second enzyme of the agmatine route of putrescine biosynthesis in plants[J]. Front Plant Sci, 2019, 10:320.
[33] Chaudhuri MM, Ghosh B.Agmatine deiminase in rice seedlings[J]. Phytochemistry, 1985, 24(10):2433-2435.
[34] 庄海燕, 安锋, 张硕新, 等. 乙烯利刺激橡胶树增产机制研究进展[J]. 林业科学, 2010, 46(4):120-125.
[35] 段翠芳, 曾日中, 黎瑜. 激素对巴西橡胶树橡胶生物合成的调控[J]. 热带农业科学, 2004, 24(5):61-68.
[36] 袁红梅, 洪灏, 黄惜. 巴西橡胶树产排胶机理的研究进展[J]. 分子植物育种, 2015, 13(5):1151-1156.