Production of Prodigiosin Using Waste Oil from the Processing of Sophora flavescens Seeds

doi:10.13560/j.cnki.biotech.bull.1985.2019-1161

Abstract

Abstract: To improve the utilization efficiency of Sophora flavescens resources,the strains that can use the waste oil after extracting alkaloids from S. flavescens seed to produce prodigiosin were screened,and its fermentation parameters were optimized by single factor investigation and response surface optimization. UPLC-Q-TOF-MS/MS was used to analyze the purified fermented products. As the results,a strain L9 was screened and identified as Serratia marcescens by morphology and 16S rDNA sequencing. The optimal parameters were as follows：the optimal concentration of S. flavescens seed oil,beef extract and calcium chloride were 13 g/L,9.5 g/L and 0.3 g/L,respectively,and the fermentation temperature was 30℃. Under the optimal fermentation conditions,the maximum yield of prodigiosin was about 317.21 mg/L,and the yield was increased by 3.2 times. In this paper,the by-products produced in S. flavescens seed processing was taken as the research object,and its oil components were used as resources,i.e.,producing the high value-added product prodigiosin while disposing the wastes from processing S. flavescens seed,which provides a reference for the resource utilization of solid waste in the processing of seeds medicinal materials.

Key words: Sophora flavescens seed, Serratia marcescens, prodigiosin, recycling and utilization of Chinese medicine resources

NI Liang, ZHANG Sen, GUO Sheng, ZENG Fei, XU Ming-ming, DUAN Jin-ao. Production of Prodigiosin Using Waste Oil from the Processing of Sophora flavescens Seeds[J]. Biotechnology Bulletin, 2020, 36(5): 130-138.

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