Role of Alternative Respiratory Pathway in Brassinosteroids Inducing Heat Stress Response in Nicotiana benthamiana

doi:10.13560/j.cnki.biotech.bull.1985.2020-0591

Abstract

Abstract:

Brassinosteroids（BRs）,a class of steroid hormones,play important roles in regulation of plant growth and development,as well as the response to stress. To date,the crosstalk between BR and mitochondria and signaling mechanism of BR in regulating mitochondria under stress remain largely unknown. In this study,the role of mitochondrial alternative respiratory pathway in BR inducing heat stress tolerance in Nicotiana benthamiana was investigated. External application of BR increased the resistance of N. benthamiana to heat stress,accompanied by the induction of alternative respiration and up-regulation of NbAOX1 expression. Chemical inhibition of alternative respiration or inhibition of NbAOX1 through virus-induced gene silencing assay compromised BR-induced heat resistance in N. benthamiana. In conclusion,our results indicate that the alternate respiratory pathway induced by BR can avoid the superfluous reactive oxygen species accumulation and protect the photosystems in plants under heat stress condition.

Key words: Nicotiana benthamiana, brassinosteroids, alternative respiration, heat stress

ZHANG Rui-ping, YANG Feng, CHEN Le-zhang, DENG Xing-guang, ZHANG Da-wei. Role of Alternative Respiratory Pathway in Brassinosteroids Inducing Heat Stress Response in Nicotiana benthamiana[J]. Biotechnology Bulletin, 2020, 36(10): 8-14.

Figures/Tables 4

References 24

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