Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (10): 127-134.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0236

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Isolation,Identification and Characterization of Salt-alkali-tolerant and Phosphorus-dissolving Bacterium Y2R2

YANG Hai-xia(), LIU Xi-min, PAN Yi-chen, ZHAO Xiang-lin, HUANG Hai-dong()   

  1. College of Agronomy & Resources and Environment,Tianjin Agricultural University,Tianjin 300384
  • Received:2020-03-07 Online:2020-10-26 Published:2020-11-02
  • Contact: HUANG Hai-dong E-mail:yhx0118@126.com;hdhuang@tjau.edu.cn

Abstract:

The objective of this study is to screen phosphorus-dissolving bacteria with fine environment adaptability to coastal salt-alkali land. Strain Y2R2 with the ability of salt-alkali-tolerant and phosphorus-dissolving was isolated from salt-alkali soil samples collected from Tianjin Binhai New Area. Strain Y2R2 was identified as a novel species of Halomonas according to poly-phase taxonomic characteristics. Strain Y2R2 grew at the temperature 10℃ to 45℃,and tolerated the condition of pH 14 and 15% NaCl. Strain Y2R2 showed ability of dissolving phosphorus under pH 7-13 and NaCl content 0-12%. In inorganic phosphorus medium,under the fermentation conditions in which the initial pH was 8,NaCl content was 9% and fermentation time was 48 h,the phosphate-dissolving quantity by strain Y2R2 reached its highest value,247.6 mg/L. Strain Y2R2 screened in this study is a novel species of indigenous microorganisms that adapted to the soil and climate environment of coastal salt-alkali land,and has high salt-alkali tolerance and phosphate-dissolving capacities. The strain is expected to be used as salt-alkali phosphorus-dissolving bacterial agent.

Key words: salt-alkali-tolerant phosphorus dissolving bacteria, taxonomic identification, Halomonas, novel species