Research on a Preparation Process of Deoxynivalenol

doi:10.13560/j.cnki.biotech.bull.1985.2020-0325

Abstract

Abstract:

Deoxynivalenol（DON）is a mycotoxin produced by Fusarium. It is cytotoxic and can cause embryo deformity,which seriously threatens human and animal health. To provide enough high-purity DON for scientific research in toxicology and detoxification,we developed a simple,economical and effective process for the separation and purification of DON. 1. Extraction：84% acetonitrile aqueous solution was used to extract DON from the rice culture of Fusarium graminearum PH-1 to obtain DON crude extract. 2. Cleaning：Petroleum ether and saturated saline aqueous solution were used to remove the impurity from the DON crude extract. 3. Refining：The product obtained in step 2 was washed by ethyl acetate：petroleum ether (1∶1 of V/V). Then more impurity was removed by liquid-liquid distribution based on the polarity difference between ethyl acetate and saturated saline aqueous solution. 4. Purification：Helped by preparative high performance liquid chromatography DON in high purity was obtained. With this method 61.36 mg highly purified DON was obtained from one kilogram PH-1 culture. Its main feature is that the purity of the refined DON has reached 80%,which could be directly applied in conventional scientific research. If further purification needed via preparative liquid chromatography,the column loss can be remarkably reduced,therefore the economic cost decreases.

Key words: deoxynivalenol, separation and purification, Fusarium graminearum

DUAN Jia-qi, LI Bei-bei, REN Jie, QIU De-wen, LI Guang-yue. Research on a Preparation Process of Deoxynivalenol[J]. Biotechnology Bulletin, 2020, 36(10): 256-262.

Figures/Tables 7

References 27

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指标	乙腈-水（84:16,V/V）	甲醇-水（9:1,V/V）	甲醇-水（8:2,V/V）	甲醇-水（7:3,V/V）
提取率/%	100	22.7	96.9	102
DON/粗毒素/%	2.9	0.5	2.2	1.9

指标	乙腈-水（84:16,V/V）	甲醇-水（9:1,V/V）	甲醇-水（8:2,V/V）	甲醇-水（7:3,V/V）
提取率/%	100	22.7	96.9	102
DON/粗毒素/%	2.9	0.5	2.2	1.9