Cloning,Expression and Bioinformatics Analyses of HbCPA Gene from Hevea brasiliensis

doi:10.13560/j.cnki.biotech.bull.1985.2020-0294

Abstract

Abstract:

N-carbamoylputreseine amidohydrolase（CPA）is the final reaction enzyme for higher plants to synthesize putrescine through the arginine pathway. A CPA gene from Hevea brasiliensis was cloned with RACE and RT-PCR and designated as HbCPA. The full length of HbCPA was 1 342 bp with an open reading frame of 906 bp and encoded 301 amino acids. The molecular weight and isoelectric point of HbCPA were 33.5 kD and 6.01,respectively. Phylogenetic analyses showed that HbCPA and MeCPA in Manihot esculenta,RcCPA in Ricinus communis and JcCPA in Jatropha curcas clustered into one branch. qRT-PCR analyses demonstrated that HbCPA expressed in each tissue,with the highest expression in barks,followed by leaves,female flowers,male flowers and stem apexes. The expression of HbCPA varied in different developmental stages of leaves,with the highest in the light green period,and the same and lowest expression in the bronze and senescence stages. The expression of HbCPA was significantly up-regulated in barks of tapping panel dryness（TPD）tree compared with the healthy rubber tree. HbCPA expression was also regulated by ethephon,MeJA,low temperature,drought,high salt and hydrogen peroxide. All these results suggest that HbCPA may be involved in the regulation of rubber production and debinding in rubber tree and the response to abiotic stress.

Key words: Hevea brasiliensis, N-carbamoylputreseine amidohydrolase, abiotic stress, bioinformatics analysis, expression analysis

YANG Hong, HU Yan-ling, YUE Yi-fan, DENG Zhi, DAI Long-jun, LI De-jun. Cloning,Expression and Bioinformatics Analyses of HbCPA Gene from Hevea brasiliensis[J]. Biotechnology Bulletin, 2020, 36(11): 39-47.

Figures/Tables 5

References 28

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