Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (11): 30-38.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0200

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Identification and Antifungal Activity Determination of an Endophytic Fungus from the Twigs of Vaccinium dunalianum

YAN Dong1,2(), ZENG Wei-lin1,2, LUO Xu-lu1,2, CHEN Xiao-xue2,3, LIU Hui-min2,3, ZHAO Ping1,2,3()   

  1. 1. Key Laboratory of State Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming 650224
    2. Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China,Southwest Forestry University,Ministry of Education,Kunming 650224
    3. Collaborative Innovation Center of Forest Resources Breeding and Utilization in Yunnan,Kunming 650224
  • Received:2020-02-29 Online:2020-11-26 Published:2020-11-20
  • Contact: ZHAO Ping E-mail:1822545197@qq.com;hypzhao@yahoo.com

Abstract:

An endophytic fungal strain Z-78 isolated from the twigs of Vaccinium dunalianum Wight was identified as Pestalotiopsis cocculi(Guba)G.C. Zhao & N. Li by morphological classification and molecular biological study. The plate confrontation method was used to determine its antifungal activities against eight plant pathogens including Botrytis cinerea,Fusarium solani,Fusarium graminearum,Fusarium oxysporum,Alternaria brassicicola,Gloeophyllum trabeum,Poria placenta and Coriolus versicolor,and the growth rate method was applied to conduct the acid-base stability test of the fermentation broth inhibiting the activity of A. brassicicola. The results showed that Z-78 strain presented strong inhibitory effects on all of the eight plant pathogens,while the activity against A. brassicicola remained stable under different pH conditions of fermentation broth.

Key words: Vaccinium dunalianum, twig, endophytic fungi, Pestalotiopsis cocculi, antifungal activity