Study on the Antibacterial Mechanism of Alpinetin Against Fish-derived Drug-resistant Aeromonas hydrophila in vitro

doi:10.13560/j.cnki.biotech.bull.1985.2020-0743

Abstract

Abstract:

The aim of this study is to investigate the antibacterial effect and the mechanism of alpinetin against drug-resistant Aeromonas hydrophila in vitro. We investigated the mechanism of alpinetin against drug-resistant A. hydrophila by measuring the effects of alpinetin on A. hydrophila cell growth,cell morphology,electrical conductivity,lactate dehydrogenase（LDH）,protein metabolism and DNA. The minimal inhibitory concentration（MIC）and minimum bactericidal concentration（MBC）of alpinetin against 5 strains of fish-derived A. hydrophila were 128-256 μg/mL and 512-1 024 μg/mL,respectively. Compared with the control group,after treating A. hydrophila with 2 MIC alpinetin for 8 or 16 h,the bacteria showed shrinkage,disrupted cell wall and membrane,loss of cytoplasm,and interior cavitation. After treating for 2 h,bacteria suspension’s electrical conductivity,LDH activity content and DNA extravasation increased by 3.97%,26.09% and 10.50 μg/mL,respectively. After treating for 2,4 and 8 h,nucleic acid fluorescence intensity and density in the bacterial suspension showed a time-dependent decreasing tendency. The in vitro antibacterial mechanism of alpinetin against A. hydrophila is to inhibit their growth and reproduction mainly via destroying cell walls of bacteria and increasing the permeability of the cell membrane.

Key words: alpinetin, Aeromonas hydrophila, antibacterial mechanism, cell morphology

CHEN Jie-hao, MIAO Yu-jia, LIANG Chao, TAO Yu, OUYANG Ping, WANG Kai-yu, GENG Yi, SHI Cun-bin, LI Ning-qiu. Study on the Antibacterial Mechanism of Alpinetin Against Fish-derived Drug-resistant Aeromonas hydrophila in vitro[J]. Biotechnology Bulletin, 2021, 37(2): 103-110.

Figures/Tables 8

References 38

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嗜水气单胞菌名称	来源	耐药性^*	山姜素
嗜水气单胞菌名称	来源	耐药性^*	MIC/（μg·mL^-1）	MBC/（μg·mL^-1）
CW	斑点叉尾鮰	GEN,NEO,TE,DO,AML	128	256
1G	鲫鱼	ROX,RFP,AML	256	512
Ah	长吻鮠	GEM,FFC,DO,AML	256	1024
WZ1	黄颡鱼	ROX,RFP,AML	128	512
S1D	鳜鱼	GEM,AML	256	512

嗜水气单胞菌名称	来源	耐药性^*	山姜素
嗜水气单胞菌名称	来源	耐药性^*	MIC/（μg·mL^-1）	MBC/（μg·mL^-1）
CW	斑点叉尾鮰	GEN,NEO,TE,DO,AML	128	256
1G	鲫鱼	ROX,RFP,AML	256	512
Ah	长吻鮠	GEM,FFC,DO,AML	256	1024
WZ1	黄颡鱼	ROX,RFP,AML	128	512
S1D	鳜鱼	GEM,AML	256	512