Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (2): 80-87.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0848

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miR-378 Promoting Lipogenesis and Identification of Target Genes

ZHANG Ting-huan(), LONG Xi, GUO Zong-yi, CHAI Jie()   

  1. 1. Chongqing Academy of Animal Science,Chongqing 402460
    2. Key Laboratory of Pig Industry Sciences of Ministry of Agriculture,Chongqing 402460
  • Received:2020-07-09 Online:2021-02-26 Published:2021-02-26
  • Contact: CHAI Jie E-mail:ztinghuan@163.com;jiechai91@163.com

Abstract:

The purpose of this study was to investigate the function of miR-378 in adipocytes and to filter and verify its target genes. 3T3-L1 cells were transfected with miR-378 mimic to validate the function of mir-378 in adipocytes. The potential target genes of miR-378 were identified according to the conservation of target sites and lipid promoting function. The relationship between miR-378 and its targets was verified by microRNA pulldown technology,and then the binding sites of miR-378 and its targets were determined by Dual-Luciferase Reporter Assay System. Some important results were found that miR-378 promoted lipogenesis in adipocytes by increasing lipid synthesis and decreasing lipid decomposition,and the relationship between miR-378 and Runx1t1,Galnt3,RAB10 and the binding sites were determined.

Key words: miR-378, lipogenesis, microRNA pulldown, target genes, target sites