Effects of Resistant and Sensitive Rice Varieties on Gut Microbiota of Nilaparvata lugens

doi:10.13560/j.cnki.biotech.bull.1985.2020-0797

Abstract

Abstract:

This study aims to investigate the effects of resistant and sensitive rice varieties on the diversity and abundance of the gut microbiota of brown planthopper（BPH）. The 3^rd instar BPH nymphs were feeding on resistant rice variety RHT,susceptible rice variety TN1,and medium-resistant rice variety ZH11,and the intestines of BPH were sampled after 1 and 3 d. Total DNA were extracted and sequenced through the HiSeq 2500 platform for 16S rRNA-V4 region of bacteria in the gut. The sequencing results were analyzed to count the number of OTUs（Operational taxonomic unit）in different samples. The species composition,abundance,and the alpha diversity were counted at different levels of taxonomies. The specific and common bacteria of BPH after feeding with three different rice varieties were counted. The bacteria with high abundance and significant difference were analyzed. More than 70 000 valid sequences were obtained from each sample,and a certain number of OTUs were clustered based on sequence similarity. There were 213 common OTUs and 424,77,and 130 unique OTUs in 1 d samples of BPH after feeding on RHT,TN1,and ZH11,respectively,and 217 common OTUs and 140,162,63 unique OTUs in 3 d samples. The Acinetobacter and Wolbachia were two dominant gut microbiota species,and the content of Acinetobacter in the brown planthopper that fed on RHT was lower than that fed on TN1 and ZH11. Among 319 genera of bacteria in BPH,8 types of bacteria demonstrated significant differences in BPH after feeding on RHT,TN1 and ZH11 rice,respectively,which may be closely related to the BPH feeding on different resistant rice varieties. BPH can respond to different resistant rice varieties by changing the intestinal bacterial composition. This study lays a foundation for further exploring the intestinal microbiota of the BPH and their response to different rice varieties,and also provides a reference for controlling brown planthoppers using microbial resources.

Key words: Nilaparvata lugens, resistant variety, susceptible variety, rice, 16S rRNA, gut microbiota

LI Hai-chao, XIE Fei, ZHANG Yuan-qi, GUAN Ruo-bing. Effects of Resistant and Sensitive Rice Varieties on Gut Microbiota of Nilaparvata lugens[J]. Biotechnology Bulletin, 2021, 37(3): 1-9.

Figures/Tables 4

References 36

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Sample	observed species	chao	ace	shannon	simpson	coverage
mean（RHT.1D）	388.66667	395.44461	395.11502	1.45736	0.51068	0.99972
SD（RHT.1D）	192.10501	190.41556	191.33268	0.23077	0.18074	0.00009
mean（TN1.1D）	192.33333	233.66591	267.93055	1.92246	0.31738	0.99946
SD（TN1.1D）	93.32917	65.20748	27.16002	0.99904	0.17186	0.00036
mean（ZH11.1D）	251.66667	255.6381	254.37961	2.03145	0.35986	0.99988
SD（ZH11.1D）	50.73789	51.35924	50.2521	0.50533	0.11153	0.00002
mean（RHT.3D）	253	260.98931	259.054	1.46526	0.50256	0.99977
SD（RHT.3D）	76.5441	76.10176	79.18294	0.34932	0.08544	0.00014
mean（TN1.3D）	282.33333	296.16628	292.6945	1.62864	0.37817	0.99963
SD（TN1.3D）	39.10669	43.00768	41.60751	0.04288	0.07577	0.00015
mean（ZH11.3D）	181	216.55371	220.79586	0.91988	0.57233	0.99945
SD（ZH11.3D）	93.95212	61.23732	58.88241	0.57029	0.28043	0.00014

Sample	observed species	chao	ace	shannon	simpson	coverage
mean（RHT.1D）	388.66667	395.44461	395.11502	1.45736	0.51068	0.99972
SD（RHT.1D）	192.10501	190.41556	191.33268	0.23077	0.18074	0.00009
mean（TN1.1D）	192.33333	233.66591	267.93055	1.92246	0.31738	0.99946
SD（TN1.1D）	93.32917	65.20748	27.16002	0.99904	0.17186	0.00036
mean（ZH11.1D）	251.66667	255.6381	254.37961	2.03145	0.35986	0.99988
SD（ZH11.1D）	50.73789	51.35924	50.2521	0.50533	0.11153	0.00002
mean（RHT.3D）	253	260.98931	259.054	1.46526	0.50256	0.99977
SD（RHT.3D）	76.5441	76.10176	79.18294	0.34932	0.08544	0.00014
mean（TN1.3D）	282.33333	296.16628	292.6945	1.62864	0.37817	0.99963
SD（TN1.3D）	39.10669	43.00768	41.60751	0.04288	0.07577	0.00015
mean（ZH11.3D）	181	216.55371	220.79586	0.91988	0.57233	0.99945
SD（ZH11.3D）	93.95212	61.23732	58.88241	0.57029	0.28043	0.00014