Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (3): 10-17.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0861
Previous Articles Next Articles
SUN Xiao-qian(), WANG Jia-rui, CHEN Qing-fu, LI Hong-you()
Received:
2020-07-13
Online:
2021-03-26
Published:
2021-04-02
Contact:
LI Hong-you
E-mail:sunxiaoqianxqs@163.com;lihongyouluod@163.com
SUN Xiao-qian, WANG Jia-rui, CHEN Qing-fu, LI Hong-you. Gene Cloning,Subcellular Localization and Expression Analyses of FtMYBF Transcription Factor in Fagopyrum tataricum[J]. Biotechnology Bulletin, 2021, 37(3): 10-17.
引物名称 | 引物序列(5'-3') |
---|---|
FtMYBF-f1 | ATGGGAAGAGCACCTTGCTG |
FtMYBF-r1 | CTCAAGAAAGAAGCCAGGCAAC |
FtMYBF-f2 | CAAGCTTGCATGCCTGCAGGTCGACATGGGAA- GAGCACCTTGCTG |
FtMYBF-r2 | CCTCGCCCTTGCTCACCATGGATCCAGAAAGA- AGCCAGGCAACCA |
FtMYBF-qf | GGGAGAGAGTAGCTGCAATG |
FtMYBF-qr | CCCACATTAGCCACGATAGG |
FtActin7-f | ATGTTCACTACCACCGCTGA |
FtActin7-r | TGAACCTCTCAGCACCAATC |
引物名称 | 引物序列(5'-3') |
---|---|
FtMYBF-f1 | ATGGGAAGAGCACCTTGCTG |
FtMYBF-r1 | CTCAAGAAAGAAGCCAGGCAAC |
FtMYBF-f2 | CAAGCTTGCATGCCTGCAGGTCGACATGGGAA- GAGCACCTTGCTG |
FtMYBF-r2 | CCTCGCCCTTGCTCACCATGGATCCAGAAAGA- AGCCAGGCAACCA |
FtMYBF-qf | GGGAGAGAGTAGCTGCAATG |
FtMYBF-qr | CCCACATTAGCCACGATAGG |
FtActin7-f | ATGTTCACTACCACCGCTGA |
FtActin7-r | TGAACCTCTCAGCACCAATC |
[1] |
Zhang LJ, Li XX, Ma B, et al. The tartary buckwheat genome provides insights into rutin biosynjournal and abiotic stress tolerance[J] . Molecular Plant, 2017,10(9):1224-1237.
doi: 10.1016/j.molp.2017.08.013 URL pmid: 28866080 |
[2] |
Zhu F. Chemical composition and health effects of tartary buckwheat[J] . Food Chemistry, 2016,203:231-245.
doi: 10.1016/j.foodchem.2016.02.050 URL pmid: 26948610 |
[3] |
Li J, Yang P, Yang Q, et al. Analysis of flavonoid metabolites in buckwheat leaves using UPLC-ESI-MS/MS[J] . Molecules, 2019,24(7):1310.
doi: 10.3390/molecules24071310 URL |
[4] |
Li H, Lv Q, Ma C, et al. Metabolite profiling and transcriptome analyses provide insights into the flavonoid biosynjournal in the developing seed of tartary buckwheat(Fagopyrum tataricum)[J] . Journal of Agricultural and Food Chemistry, 2019,67(40):11262-11276.
doi: 10.1021/acs.jafc.9b03135 URL pmid: 31509416 |
[5] |
Nabavi SM, Šamec D, Tomczyk M, et al. Flavonoid biosynthetic pathways in plants:versatile targets for metabolic engineering[J] . Biotechnology Advances, 2020,38:107316.
doi: 10.1016/j.biotechadv.2018.11.005 URL pmid: 30458225 |
[6] | Afrin S, Nuruzzaman M, Zhu J, et al. Combinatorial interactions of MYB and bHLH in flavonoid biosynjournal and their function in plants[J] . Journal of Plant Biology Research, 2014,3(2):65-77. |
[7] |
Li S. Transcriptional control of flavonoid biosynjournal:fine-tuning of the MYB-bHLH-WD40(MBW)complex[J] . Plant Signaling & Behavior, 2014,9(1):e27522.
URL pmid: 24393776 |
[8] |
Wen W, Alseekh S, Fernie AR. Conservation and diversification of flavonoid metabolism in the plant kingdom[J] . Current Opinion in Plant Biology, 2020,55:100-108.
URL pmid: 32422532 |
[9] | 邢文, 金晓玲. 调控植物类黄酮生物合成的MYB转录因子研究进展[J] . 分子植物育种, 2015,13(3):689-696. |
Xing W, Jin XL. Recent advances of MYB transcription factors involved in the regulation of flavonoid biosynjournal[J] . Molecular Plant Breeding, 2015,13(3):689-696. | |
[10] | 温东, 师玉华, 赵锐, 等. 苦荞MYB家族转录因子研究进展[J] . 中国实验方剂学杂志, 2019,25(6):227-234. |
Wen D, Shi YH, Zhao R, et al. MYB transcription factors in Fagopyrum tataricum[J] . Chinese Journal of Experimental Traditional Medical Formulae, 2019,25(6):227-234. | |
[11] |
Bai YC, Li CL, Zhang JW, et al. Characterization of two tartary buckwheat R2R3-MYB transcription factors and their regulation of proanthocyanidin biosynjournal[J] . Physiologia Plantarum, 2014,152(3):431-440.
doi: 10.1111/ppl.12199 URL |
[12] |
Luo XP, Zhao HX, Yao PF, et al. An R2R3-MYB transcription factor FtMYB15 involved in the synjournal of anthocyanin and proanthocyanidins from tartary buckwheat[J] . Journal of Plant Growth Regulation, 2018,37(1):76-84.
doi: 10.1007/s00344-017-9709-3 URL |
[13] | 余艳, 廖小龙, 肖玉华, 等. 苦荞转录因子FtMYB56基因的克隆及其对黄酮合成的调控[J/OL] . 基因组学与应用生物学. http//kns.cnki.net/kcms/detail/45.1369.q,20191023.1519.004.html. |
Yu Y, Liao XH, Xiao YH, et al. Transcription factors FtMYB56 cloned from Fagopyrum tataricum, its effects on flavonoid biosynthesis[J/OL] . Genomics and Applied Biology. http//kns.cnki.net/kcms/detail/45.1369.q,20191023.1519.004.html. | |
[14] |
Zhang D, Jiang CL, Huang CH, et al. The light-induced transcription factor FtMYB116 promotes accumulation of rutin in Fagopyrum tataricum[J] . Plant Cell and Environment, 2019,42(4):1340-1351.
doi: 10.1111/pce.v42.4 URL |
[15] |
Sun ZX, Linghu B, Hou SY, et al. Tartary buckwheat FtMYB31 gene encoding an R2R3-MYB transcription factor enhances flavonoid accumulation in tobacco[J] . Journal of Plant Growth Regulation, 2020,39:564-574.
doi: 10.1007/s00344-019-10000-7 URL |
[16] |
Luo XP, Li SJ, Yao PF, et al. The jasmonate-ZIM domain protein FtJAZ2 interacts with the R2R3-MYB transcription factor FtMYB3 to affect anthocyanin biosynjournal in tartary buckwheat[J] . Turkish Journal of Biology, 2017,41:526-534.
doi: 10.3906/biy-1610-6 URL |
[17] |
Huang YJ, Wu Q, Wang S, et al. FtMYB8 from Tartary buckwheat inhibits both anthocyanin/proanthocyanidin accumulation and marginal trichome initiation[J] . BMC Plant Biology, 2019,19:263.
doi: 10.1186/s12870-019-1876-x URL pmid: 31215400 |
[18] |
Zhou ML, Sun ZM, Ding MQ, et al. FtSAD2 and FtJAZ1 regulate activity of the FtMYB11 transcription repressor of the phenylpropanoid pathway in Fagopyrum tataricum[J] . New Phytologist, 2017,216(3):814-828.
doi: 10.1111/nph.2017.216.issue-3 URL |
[19] |
Zhang KX, Logacheva MD, Meng Y, et al. Jasmonate-responsive MYB factors spatially repress rutin biosynjournal in Fagopyrum tataricum[J] . Journal of Experimental Botany, 2018,69(8):1955-1966.
doi: 10.1093/jxb/ery032 URL pmid: 29394372 |
[20] |
Li JB, Zhang KX, Meng Y, et al. FtMYB16 interacts with Ftimportin-α1 to regulate rutin biosynjournal in tartary buckwheat[J] . Plant Biotechnology Journal, 2019,17:1479-1481.
doi: 10.1111/pbi.13121 URL pmid: 30963665 |
[21] | 伍小方, 高国应, 左倩, 等. FtMYB1转录因子调控苦荞毛状根黄酮醇合成的机理研究[J] . 植物遗传资源学报, 2020,21(5):1270-1278. |
Wu XF, Gao GY, Zuo Q, et al. Deciphering the functional basis of FtMYB1 transcription factor in flavonol biosynjournal of tartary buckwheat hairy root[J] . Journal of Plant Genetic Resources, 2020,21(5):1270-1278. | |
[22] |
Yao P, Zhao H, Luo X, et al. Chalcone synthase homologous genes cloning and expression pattern in flowering Fagopyrum tataricum Gaertn[J] . Russian Journal of Plant Physiology, 2016,63:790-799.
doi: 10.1134/S1021443716060169 URL |
[23] | 王璐瑗, 荣玉萍, 黄娟, 等. 211份金荞麦收集系根茎黄酮含量的分析评价[J] . 贵州师范大学学报:自然科学版, 2019,37(4):25-30, 48. |
Wang LY, Rong YP, Huang J, et al. Analysis and evaluation of the flavonoid content of rhizomes of 211 different Golden buckwheat accessions(Fagopyrum cymosum complex)[J] . Journal of Guizhou Normal University:Natural Sciences, 2019,37(4):25-30, 48. | |
[24] |
Huang W, Sun W, Lv H, et al. Isolation and molecular characterization of thirteen R2R3-MYB transcription factors from Epimedium sagittatum[J] . International Journal of Molecular Sciences, 2012,14(1):594-610.
doi: 10.3390/ijms14010594 URL pmid: 23271373 |
[25] |
Stracke R, Werber M, Weisshaar B. The R2R3-MYB gene family in Arabidopsis thaliana[J] . Current Opinion in Plant Biology, 2001,4(5):447-456.
doi: 10.1016/S1369-5266(00)00199-0 URL |
[26] |
Czemmel S, Stracke R, Weisshaar B, et al. The grapevine R2R3-MYB transcription factor VvMYBF1 regulates flavonol synjournal in developing grape berries[J] . Plant Physiology, 2009,151(3):1513-1530.
doi: 10.1104/pp.109.142059 pmid: 19741049 |
[27] |
Matsui K, Oshima Y, Mitsuda N, et al. Buckwheat R2R3 MYB transcription factor FeMYBF1 regulates flavonol biosynjournal[J] . Plant Science, 2018,274:466-475.
doi: 10.1016/j.plantsci.2018.06.025 URL pmid: 30080636 |
[28] |
Mehrtens F, Kranz H, Bednarek P, et al. The Arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynjournal[J] . Plant Physiology, 2005,138(2):1083-1096.
doi: 10.1104/pp.104.058032 URL pmid: 15923334 |
[29] | Stracke R, Ishihara H, Huep G, et al. Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling[J] . The Plant Journal, 2007,509(4):660-677. |
[30] |
Wang N, Xu H, Jiang S, et al. MYB12 and MYB22 play essential roles in proanthocyanidin and flavonol synjournal in red-fleshed apple(Malus sieversii f. niedzwetzkyana)[J] . The Plant Journal, 2017,90(2):276-292.
URL pmid: 28107780 |
[31] |
Zhai R, Zhao Y, Wu M, et al. The MYB transcription factor PbMYB12b positively regulates flavonol biosynjournal in pear fruit[J] . BMC Plant Biology, 2019,19(1):85.
doi: 10.1186/s12870-019-1687-0 pmid: 30791875 |
[32] |
Liu C, Long J, Zhu K, et al. Characterization of a citrus R2R3-MYB transcription factor that regulates the flavonol and hydroxycinnamic acid biosynjournal[J] . Scientific Reports, 2016,6:25352.
doi: 10.1038/srep25352 URL pmid: 27162196 |
[1] | LYU Qiu-yu, SUN Pei-yuan, RAN Bin, WANG Jia-rui, CHEN Qing-fu, LI Hong-you. Cloning, Subcellular Localization and Expression Analysis of the Transcription Factor Gene FtbHLH3 in Fagopyrum tataricum [J]. Biotechnology Bulletin, 2023, 39(8): 194-203. |
[2] | WANG Jia-rui, SUN Pei-yuan, KE Jin, RAN Bin, LI Hong-you. Cloning and Expression Analyses of C-glycosyltransferase Gene FtUGT143 in Fagopyrum tataricum [J]. Biotechnology Bulletin, 2023, 39(8): 204-212. |
[3] | LI Bo, LIU He-xia, CHEN Yu-ling, ZHOU Xing-wen, ZHU Yu-lin. Cloning, Subcellular Localization and Expression Analysis of CnbHLH79 Transcription Factor from Camellia nitidissima [J]. Biotechnology Bulletin, 2023, 39(8): 241-250. |
[4] | SUN Ming-hui, WU Qiong, LIU Dan-dan, JIAO Xiao-yu, WANG Wen-jie. Cloning and Expression Analysis of CsTMFs Gene in Tea Plant [J]. Biotechnology Bulletin, 2023, 39(7): 151-159. |
[5] | ZHANG He-chen, YUAN Xin, GAO Jie, WANG Xiao-chen, WANG Hui-juan, LI Yan-min, WANG Li-min, FU Zhen-zhu, LI Bao-yin. Mechanism of Flower Petal Coloration and Molecular Breeding [J]. Biotechnology Bulletin, 2023, 39(5): 23-31. |
[6] | MA Fang-fang, LIU Guan-wen, PANG Bing, JIANG Chun-mei, SHI Jun-ling. Strategies of Increasing Flavonoid Production in Engineered Bacteria by Intensifying the Efflux of Flavonoid in Cells [J]. Biotechnology Bulletin, 2023, 39(5): 63-76. |
[7] | LIU Si-jia, WANG Hao-nan, FU Yu-chen, YAN Wen-xin, HU Zeng-hui, LENG Ping-sheng. Cloning and Functional Analysis of LiCMK Gene in Lilium ‘Siberia’ [J]. Biotechnology Bulletin, 2023, 39(3): 196-205. |
[8] | YANG Xu-yan, ZHAO Shuang, MA Tian-yi, BAI Yu, WANG Yu-shu. Cloning of Three Cabbage WRKY Genes and Their Expressions in Response to Abiotic Stress [J]. Biotechnology Bulletin, 2023, 39(11): 261-269. |
[9] | HOU Rui-ze BAO Yue CHEN Qi-liang MAO Gui-ling WEI Bo-lin HOU Lei-ping LI Mei-lan. Cloning,Expression and Functional Identification of PRR5 Gene in Pakchoi [J]. Biotechnology Bulletin, 2023, 39(10): 128-135. |
[10] | QI Fang-ting, HUANG He. Research Advance in the Regulation Mechanism of Flower Spots Formation in Ornamental Plant [J]. Biotechnology Bulletin, 2023, 39(10): 17-28. |
[11] | YANG Min, LONG Yu-qing, ZENG Juan, ZENG Mei, ZHOU Xin-ru, WANG Ling, FU Xue-sen, ZHOU Ri-bao, LIU Xiang-dan. Cloning and Function Analysis of Gene UGTPg17 and UGTPg36 in Lonicera macranthoides [J]. Biotechnology Bulletin, 2023, 39(10): 256-267. |
[12] | GUAN Zhi-xiu, WANG Yan, LIANG Cheng-gang, WEI Chun-yu, HUANG Juan, CHEN Qing-fu. Identification of FtCBL Genes in Fagopyrum tataricum and Their Stress Responses to Drought and High Calcium [J]. Biotechnology Bulletin, 2022, 38(8): 101-109. |
[13] | GUO Zhi-hao, JIN Ze-xin, LIU Qi, GAO Li. Bioinformatics Analysis, Subcellular Localization and Toxicity Verification of Effector g11335 in Tilletia contraversa Kühn [J]. Biotechnology Bulletin, 2022, 38(8): 110-117. |
[14] | LI Xiu-qing, HU Zi-yao, LEI Jian-feng, DAI Pei-hong, LIU Chao, DENG Jia-hui, LIU Min, SUN Ling, LIU Xiao-dong, LI Yue. Cloning and Functional Analysis of Gene GhTIFY9 Related to Cotton Verticillium Wilt Resistance [J]. Biotechnology Bulletin, 2022, 38(8): 127-134. |
[15] | YANG Jia-bao, ZHOU Zhi-ming, ZHANG Zhan, FENG Li, SUN Li. Cloning,Expression of Helianthus annuus HaLACS1 Gene and Identification of Its Functional Complementation in Saccharomyces cerevisiae [J]. Biotechnology Bulletin, 2022, 38(6): 147-156. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||