Advance in the Research of the Antifungal Peptides

doi:10.13560/j.cnki.biotech.bull.1985.2020-0707

Abstract

Abstract:

The harmful fungi have caused plant fungal disease,food contamination and human fungal infection,which can bring great harm to people’s life and production. Moreover,the increasing resistance of harmful fungi to antifungal drugs has made them difficult to control. Due to the drug residues and the toxic effects,the traditional synthetic fungicides have been unable to meet the people’s needs. Antifungal peptides,as the natural defense molecules of living organisms,have become an important research object in response to fungal hazards and drug resistance. Antifungal peptides can inhibit the harmful fungi,and have the characteristics of high efficiency,broad spectrum and high safety. The unique antifungal mechanism of antifungal peptides can solve the problem of fungal drug resistance,which has gradually attracted people’s attention in fungi control. Based on this,this paper mainly analyzed and discussed the following aspects such as the harm of fungi,the source of antifungal peptides,the method of isolation and purification,the antifungal mechanism of antifungal peptides,etc.,aiming to provide a reference for the study of antifungal peptides.

Key words: antifungal peptides, fungal hazard, isolation and purification, antifungal mechanism

WANG Zhi-xin, LU Lei-zhen, ZHOU Jing-bo, FENG Cheng-ling, JIA Zi-wei, NING Ya-wei, JIA Ying-min. Advance in the Research of the Antifungal Peptides[J]. Biotechnology Bulletin, 2021, 37(3): 206-218.

Figures/Tables 5

References 81

[1]	燕晓翠, 杨春蕾, 姚大为, 等. 抗菌肽的国内外研究进展[J]. 天津农业科学, 2017,23(5):35-41.
	Yan XC, Yang CL, Yao DW, et al. Research progress on domestic and abroad of antibacterial peptides[J]. Tianjin Agricultural Sciences, 2017,23(5):35-41.
[2]	Choi H, Lee DG. Antifungal activity and pore-forming mechanism of astacidin 1 against Candida albicans[J]. Biochimie, 2014,105:58-63. URL pmid: 24955933
[3]	闵勇, 刘项羽, 石丽桥, 等. 细菌抗菌肽的研究及其应用前景[J]. 农村经济与科技, 2017,28(S1):179-183.
	Min Y, Liu XY, Shi L Q, et al. Research and application prospect of bacterial antimicrobial peptides[J]. Rural Economy and Science, 2017,28(S1):179-183.
[4]	贾英民, 刘杨柳, 陈洲 . 抗菌肽研究现状及其在食品安全中的应用前景[J]. 食品科学技术学报, 2017,35(6):1-9.
	Jia YM, Liu YL, Chen Z . Research status and application prospect in food safety of antimicrobial peptides[J]. Journal of Food Science and Technology, 2017,35(6):1-9.
[5]	NgTB. Peptides and proteins from fungi[J]. Peptides, 2004,25(6):1055-1073.
[6]	陈娜 . 芽孢杆菌AH-E-1抗真菌肽的分离纯化、性质及产生条件研究[D]. 天津:天津大学, 2012.
	Chen N . Isolation, purification, characterization and producing conditions of the antifungal peptides produced by Bacillus sp. AH-E-1[D]. Tianjin:Tianjin University, 2012.
[7]	Rong S, Xu H, Li L, et al. Antifungal activity of endophytic Bacillus safensis B21 and its potential application as a biopesticide to control rice blast[J]. Pesticide Biochemistry and Physiology, 2020,162:69-77.
[8]	Luz C, Izzo L, Ritieni A, et al. Antifungal and antimycotoxigenic activity of hydrolyzed goat whey on Penicillium spp:An application as biopreservation agent in pita bread[J]. LWT, 2020,118:108717.
[9]	廖子龙, 于英威, 唐坤, 等. 农作物中真菌毒素研究进展[J]. 粮油仓储科技通讯, 2019,35(2):47-49.
	Liao ZL, Yu YW, Tang K, et al. Advances in research on mycotoxin in crops[J]. LiangYouCangChuKeJiTongXun, 2019,35(2):47-49.
[10]	王鹏杰, 祁智慧, 张海洋, 等. 多元线性分析在储粮真菌生长预测中应用研究[J]. 中国粮油学报, 2020,35(1):107-112.
	Wang PJ, Qi ZH, Zhang HY, et al. Application of multiple linear analyses in prediction on growth of stored grain fungi[J]. Journal of the Chinese Cereals and Oils Association, 2020,35(1):107-112.
[11]	何晓玥, 刘栋华 . 临床常见真菌感染性皮肤病分类、致病菌生物学特征及发病机制[J]. 皮肤科学通报, 2017,34(5):512-521.
	He XY, Liu DH. Review on cutaneous fungal infection:taxonomy, biological characteristics of pathogenic fungi and pathogenesis[J]. Dermatology Bulletin, 2017,34(5):512-521.
[12]	任晓暾, 方方. 儿童中枢神经系统感染治疗疗程与腰椎穿刺检查系列建议之五——真菌性脑膜炎治疗疗程与腰椎穿刺检查建议[J]. 中国实用儿科杂志, 2020,35(1):12-15.
	Ren XT, Fang F. Course of treatment for CNS infection in children and recommendations for lumbar puncture Five- recommendations and treatment of fungal meningitis and recommendations of lumbar puncture[J]. Chinese Journal of Practical Pediatrics, 2020,35(1):12-15.
[13]	Gothe F, Schmautz A, Hausler K, et al. Treating allergic bronchopulmonary aspergillosis with short-term prednisone and itraconazole in cystic fibrosis[J]. The Journal of Allergy and Clinical Immunology:In Practice, 2020. DOI: 10.1016/j.jaip, 2020. 02. 031.
[14]	沈冰冰. 玉米茎腐病和大斑病生防菌的筛选及其促生作用的研究[D]. 哈尔滨:东北农业大学, 2019.
	Shen B . Screening of biocontrol bacteria against corn stalk rot and maize leaf blight and their growth promotion[D]. Harbin:Northeast Agricultural University, 2019.
[15]	韩乐乐, 郑丽荣 . 化学合成防腐剂的应用现状及发展趋势[J]. 科技传播, 2016,8(2):85-85, 105.
	Han L, Zheng LR. Application status and development trend of chemical synthetic preservatives[J]. Public Communication of Science & Technology, 2016,8(2):85-85, 105.
[16]	Faruck MO, Yusof F, Chowdhury S. An overview of antifungal peptides derived from insect[J]. Peptides, 2016,80:80-88.
[17]	Nicola AM, Albuquerque P, Paes HC, et al. Antifungal drugs:New insights in research & development[J]. Pharmacology & Therapeutics, 2019,195:21-38.
[18]	Mabi Chandrika KVS, Sharma S. Promising antifungal agents:A minireview[J]. Bioorganic & Medicinal Chemistry, 2020,115398.
[19]	Boman HG. Antibacterial peptides:key components needed in immunity[J]. Cell, 1991,65(2):205-207. URL pmid: 2015623
[20]	刘秀, 郭中坤, 王可洲 . 抗菌肽来源、分类方式、生物学活性、作用机制及应用研究进展[J]. 中国医药生物技术, 2016,11(6):539-543.
	Liu X, Guo ZK, Wang KZ. Research progress in sources, classification, biological activity, mechanism of action and application of antimicrobial peptides[J]. Chinese Medicinal Biotechnology, 2016,11(6):539-543.
[21]	Al Souhail Q, Hiromasa Y, Rahnamaeian M, et al. Characterization and regulation of expression of an antifungal peptide from hemolymph of an insect, Manduca sexta[J]. Developmental & Comparative Immunology, 2016,61:258-268.
[22]	Maistrou S, Paris V, Jensen AB, et al. A constitutively expressed antifungal peptide protects Tenebrio molitor during a natural infection by the entomopathogenic fungus Beauveria bassiana[J]. Developmental & Comparative Immunology, 2018,86:26-33.
[23]	丁云超, 张士璀 . 海洋动物抗菌肽研究进展[J]. 中国海洋药物, 2013,32(6):87-96.
	Ding YC, Zhang SC. Antimicrobial peptides from marine invertebrates and fish[J]. Chinese Journal of Marine Drugs, 2013,32(6):87-96.
[24]	Rose WM, Ourth DD. Isolation of lysozyme and an antifungal peptide from sea lamprey(Petromyzon marinus)plasma[J]. Veterinary Immunology and Immunopathology, 2009,132(2):264-269.
[25]	Carlos LA, Alba A, Silva ON, et al. Functional characterization of a synthetic hydrophilic antifungal peptide derived from the marine snail cenchritis muricatus[J]. Biochimie, 2012,94(4):968-974.
[26]	Choi H, Hwang JS, Lee DG. Antifungal effect and pore-forming action of lactoferricin B like peptide derived from centipede Scolopendra subspinipes mutilans[J]. Biochimica et Biophysica Acta(BBA)- Biomembranes, 2013,1828(11):2745-2750.
[27]	Park HG, Kyung SS, Lee KS, et al. Dual function of a bee(Apis cerana)inhibitor cysteine knot peptide that acts as an antifungal peptide and insecticidal venom toxin[J]. Developmental & Comparative Immunology, 2014,47(2):247-253.
[28]	Tian C, Gao B, Fang Q, et al. Antimicrobial peptide-like genes in Nasonia vitripennis:a genomic perspective[J], 2010,11:187.
[29]	Zhang Z, Zhu S. Comparative genomics analysis of five families of antimicrobial peptide-like genes in seven ant species[J]. Developmental and Comparative Immunology, 2012,38(2):262-274.
[30]	Riciluca KCT, Sayegh RSR, Melo RL, et al. Rondonin an antifungal peptide from spider(Acanthoscurria rondoniae)haemolymph[J]. Results in Immunology, 2012,2:66-71.
[31]	Lin P, Xia L, Ng TB. First isolation of an antifungal lipid transfer peptide from seeds of a Brassica species[J]. Peptides, 2007,28(8):1514-1519.
[32]	Lin P, Wong JH, Xia L, et al. Campesin, a thermostable antifungal peptide with highly potent antipathogenic activities[J]. Journal of Bioscience and Bioengineering, 2009,108(3):259-265.
[33]	白承之, 王转花, 李玉英 . 一种苦荞抗真菌肽的纯化及抑菌活性分析[J]. 食品科学, 2010,31(15):4-7.
	Bai CZ, Wang ZH, Li YY. Purification and activity of an antifungal peptide from the seeds of Tartary Buckwheat(Fagopyrum tataricum)[J]. Food Science, 2010,31(15):4-7.
[34]	Ruan JJ, Chen H, Shao JR, et al. An antifungal peptide from Fagopyrum tataricum seeds[J]. Peptides, 2011,32(6):1151-1158.
[35]	Mandal SM, Migliolo L, Franco OL, et al. Identification of an antifungal peptide from Trapa natans fruits with inhibitory effects on Candida tropicalis biofilm formation[J]. Peptides, 2011,32(8):1741-1747. URL pmid: 21736910
[36]	Wong JH, Ng TB. Gymnin, a potent defensin-like antifungal peptide from the Yunnan bean(Gymnocladus chinensis Baill)[J]. Peptides, 2003,24(7):963-968.
[37]	Ma DZ, Wang HX, Ng TB. A peptide with potent antifungal and antiproliferative activities from Nepalese large red beans[J]. Peptides, 2009,30(12):2089-2094.
[38]	Khani S, Seyedjavadi SS, Zare-Zardini H, et al. Isolation and functional characterization of an antifungal hydrophilic peptide, Skh-AMP1, derived from Satureja khuzistanica leaves[J]. Phytochemistry, 2019,164:136-143.
[39]	Rogozhin EA, Slezina MP, Slavokhotova AA, et al. A novel antifungal peptide from leaves of the weed Stellaria media L.[J]. Biochimie, 2015,116:125-132.
[40]	Wong JH, Ng TB, Wang H, et al. Cordymin, an antifungal peptide from the medicinal fungus Cordyceps militaris[J]. Phytomedicine, 2011,18(5):387-392.
[41]	Solanki DS, Kumar S, Parihar K, et al. Characterization of a novel seed protein of Prosopis cineraria showing antifungal activity[J]. International Journal of Biological Macromolecules, 2018,116:16-22.
[42]	于杰, 张荣意, 谭志琼, 等. 枯草芽孢杆菌B25抗真菌作用及抗菌蛋白的分离纯化[J]. 基因组学与应用生物学, 2016,35(3):629-634.
	Yu J, Zhang RY, Tan ZQ, et al. Studies on antifungal activity and purification of antifungal substance from Bacillus subtilis B25 strain[J]. Genomics and Applied Biology, 2016,35(3):629-634.
[43]	Ji ZL, Peng S, Chen LL, et al. Identification and characterization of a serine protease from Bacillus licheniformis W10:A potential antifungal agent[J]. International Journal of Biological Macromolecules, 2020,145:594-603.
[44]	Naing KW, Lee YS, Nguyen XH, et al. Isolation and characterization of an antimicrobial lipopeptide produced by Paenibacillus ehimensis MA2012[J]. Journal of Basic Microbiology, 2014,54:1-12.
[45]	Skouri-gargouri H, Gargouri A. First isolation of a novel thermostable antifungal peptide secreted by Aspergillus clavatus[J]. Peptides, 2008,29(11):1871-1877. URL pmid: 18687373
[46]	Wang H, Ng TB. Eryngin, a novel antifungal peptide from fruiting bodies of the edible mushroom Pleurotus eryngii[J]. Peptides, 2004,25(1):1-5.
[47]	Chu KT, Xia L, Ng TB. Pleurostrin, an antifungal peptide from the oyster mushroom[J]. Peptides, 2005,26(11):2098-2103.
[48]	程亮, 罗明明, 吴继纲, 等. 海洋放线菌Y12-26中抗真菌活性代谢产物的分离纯化与结构鉴定[J]. 中国抗生素杂志, 2017,42(8):631-638.
	Cheng L, Luo M, Wu JG, et al. Purifi cation and structure elucidation of antifungal bioactive metabolites from marine actinomycete Y12-26[J]. Chinese Journal of Antibiotics, 2017,42(8):631-638.
[49]	马桂珍, 吴少杰, 付泓润, 等. 海洋放线菌BM-2菌株抗真菌活性物质的分离纯化与结构鉴定[J]. 中国生物防治学报, 2014,30(3):393-401.
	Ma GZ, Wu SJ, Fu HR, et al. Isolation and structure elucidation of antifungal metabolites from marine actinomycete BM-2[J]. Chinese Journal of Biological Control, 2014,30(3):393-401.
[50]	钱英, 汪琨, 章小洪, 等. 解淀粉芽孢杆菌BW-13产生的抗真菌物质特性研究与初步分离纯化[J]. 浙江工业大学学报, 2012,40(1):42-45, 64.
	Qian Y, Wang K, Zhang XH, et al. Characterization and prefractionation of antifungal substance produced by Bacillus amylolique faciens BW-13[J]. Journal of Zhejiang University of Technology, 2012,40(1):42-45, 64.
[51]	夏敏杰, 张静, 黄迎春, 等. 枯草芽胞杆菌1. 88株抗真菌物质的分离纯化与性质研究[J]. 现代预防医学, 2010,37(16):3108-3111, 3117.
	Xia MJ, Zhang J, Huang YC, et al. Study on the mix culture and character of antifungal composition from Bacillus subtilis strain 1. 88[J]. Modern Preventive Medicine, 2010,37(16):3108-3111, 3117.
[52]	Zhao X, Zhou ZJ, Han Y, et al. Isolation and identification of antifungal peptides from Bacillus BH072, a novel bacterium isolated from honey[J]. Microbiological Research, 2013,168(9):598-606. doi: 10.1016/j.micres.2013.03.001 URL pmid: 23545354
[53]	Rong S, Xu H, Li L, et al. Antifungal activity of endophytic Bacillus safensis B21 and its potential application as a biopesticide to control rice blast[J]. Pesticide Biochemistry and Physiology, 2020,162:69-77.
[54]	严海娇, 贠建民, 白杰, 等. 短小芽孢杆菌HN-10抗菌肽的分离纯化及其抗粉红单端孢活性[J]. 食品科学, 2018,39(22):123-128.
	Yan HJ, Yun JM, Bai J, et al. Purification of an antimicrobial peptide from Bacillus pumilus HN-10 and its inhibitory activity against Trichothecium roseum[J]. Food Science, 2018,39(22):123-128.
[55]	Garibotto F M, Garro A D, Masman M F, et al. New small-size peptides possessing antifungal activity[J]. Bioorganic & Medicinal Chemistry, 2010,18(1):158-167.
[56]	罗振华, 吴建伟, 付萍, 等. 人工合成家蝇抗真菌肽MAF-1A体外抗真菌效果及扫描电镜观察[J]. 时珍国医国药, 2014,25(3):532-536.
	Luo ZH, Wu JW, Fu P, et al. Antifungal effect and scanning electron microscopy for the synthetic Musca domestica Antifungal Peptide-1A(MAF-1A)[J]. Lishizhen Medicine and Materia Medica Research, 2014,25(3):532-536.
[57]	陈明明. 家蝇抗真菌肽MAF-1A的分子改良及抗白色念珠菌活性的研究[D]. 贵阳:贵州医科大学, 2016.
	Chen MM. Study on the molecular modification and anti-canidia albicans activity of Musca domestica Antifungal Peptide-1A(MAF-1A)[D]. Guiyang:Guizhou Medical University, 2016.
[58]	Fernandez Bidondo L, Almasia N, Bazzini A, et al. The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis[J]. Crop Protection, 2019,124:104837.
[59]	Banzet N, Latorse MP, Bulet P, et al. Expression of insect cystein-rich antifungal peptides in transgenic tobacco enhances resistance to a fungal disease[J]. Plant Science, 2002,162(6):995-1006.
[60]	Sang YX, Deng XJ, Yang WY, et al. Secretive expression of insect antifungal peptide-encoded genes in Pichia pastoris and activity assay of the products[J]. Agricultural Sciences in China, 2007,6(10):1209-1216.
[61]	薛辉, 涂勇刚, 熊春红, 等. 抗菌肽快速筛选方法的研究进展[J]. 食品科学, 2019,40(13):334-339.
	Xue H, Tu YG, Xiong CH, et al. Recent progress in rapid screening methods for antibacterial peptides[J]. Food Science, 2019,40(13):334-339.
[62]	唐文婷. 基于肽—膜相互作用的模拟细胞膜法筛选抗菌肽的研究[D]. 无锡:江南大学, 2014.
	Tang WT. Research of screening for antimicrobial peptides by mimic cell membrane based on peptide-membrane interaction[D]. Wuxi:Jiangnan University, 2014.
[63]	Marie K, Hana S. High-throughput fluorescence screening assay for the identification and comparison of antimicrobial peptides’ activity on various yeast species[J]. Journal of Biotechnology, 2016,233:26-33.
[64]	Muhialdin BJ, Hassan Z, Bakar FA, et al. Identification of antifungal peptides produced by Lactobacillus plantarum IS10 grown in the MRS broth[J]. Food Control, 2016,59:27-30.
[65]	Muhialdin BJ, Algboory HL, Kadum H, et al. Antifungal activity determination for the peptides generated by Lactobacillus plantarum TE10 against Aspergillus flavus in maize seeds[J]. Food Control, 2020,109:106898.
[66]	辛磊, 李秀玲, 安慧, 等. 枯草芽孢杆菌CP6抗真菌活性物质的分离纯化[J]. 广西蚕业, 2018,55(2):12-17.
	Xin L, Li XL, An H, et al. Isolation and purification of the antifungal substance activity of Bacillus subtilis CP6[J]. Guangxi Sericulture, 2018,55(2):12-17.
[67]	孟慧云, 杨渊, 王欣荣, 等. 丝状真菌SIIA-F1108产生抗真菌物质的分离纯化和结构鉴定[J]. 中国抗生素杂志, 2015,40(12):901-905.
	Meng HY, Yang Y, Wang XR, et al. Isolation and structure identification of antifungal compounds produced by the filamentous fungi SIIA-F1108[J]. Chinese Journal of Antibiotics, 2015,40(12):901-905.
[68]	Mandal SM, Porto WF, Dey P, et al. The attack of the phytopathogens and the trumpet solo:Identification of a novel plant antifungal peptide with distinct fold and disulfide bond pattern[J]. Biochimie, 2013,95(10):1939-1948.
[69]	文超 . 海洋桔青霉W1抗真菌蛋白的分离纯化与特性研究[D]. 厦门:厦门大学, 2014.
	Wen C. Purification and characterization of an antifungal protein secreted by Penicillium citrinum W1[D]. Xiamen:Xiamen University, 2014.
[70]	张宇, 姜宁, 张爱忠 . 抗菌肽抑菌机理及其研究方法[J]. 现代畜牧兽医, 2018(1):52-57.
	Zhang Y, Jiang N, Zhang AZ. Bacteriostatic mechanism and research methods of antibacterial peptides[J]. Modern Journal of Animal Husbandry and Veterinary Medicine, 2018(1):52-57.
[71]	Mizuhara N, Kuroda M, Ogita A, et al. Antifungal thiopeptide cyclothiazomycin B1 exhibits growth inhibition accompanying morphological changes via binding to fungal cell wall chitin[J]. Bioorganic & Medicinal Chemistry, 2011,19(18):5300-5310.
[72]	高霞 . 抗真菌肽APS的制备、生物活性及其抗真菌机理研究[D]. 成都:四川大学, 2005.
	Gao X. Study on the preparation, biological activity and antifungal mechanism of antifungal peptide APS[D]. Chengdu:Sichuan University, 2005.
[73]	Boheim G. Statistical analysis of alamethicin channels in black lipid membranes[J]. The Journal of Membrane Biology, 1974,19(3):277-303. URL pmid: 4475108
[74]	Shai Y, Oren Z. From “carpet” mechanism to de-novo designed diastereomeric cell-selective antimicrobial peptides[J]. Peptides, 2001,22(10):1629-1641. doi: 10.1016/s0196-9781(01)00498-3 URL pmid: 11587791
[75]	Shai Y. Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by α-helical antimicrobial and cell non-selective membrane-lytic peptides[J]. Biochimica et Biophysica Acta(BBA)- Biomembranes, 1999,1462(1):55-70.
[76]	Jin PF, Haonan W, Zheng T, et al. Antifungal mechanism of bacillomycin D from Bacillus velezensis HN-2 against Colletotrichum gloeosporioides Penz[J]. Pesticide Biochemistry and Physiology, 2020,163:102-107.
[77]	Han Y, Zhao J, Zhang B, et al. Effect of a novel antifungal peptide P852 on cell morphology and membrane permeability of Fusarium oxysporum[J]. Biochimica et Biophysica Acta(BBA)- Biomembranes, 2019,1861(2):532-539.
[78]	Lee MR, Raman N, Ortiz-Bermúdez, P, et al. 14-Helical β-Peptides elicit toxicity against C. albicans by forming pores in the cell membrane and subsequently disrupting intracellular organelles[J]. Cell Chemical Biology, 2019,26(2):289-299.
[79]	Victoria B, Alicia R, Alberto M, et al. Development of a multiplex qPCR method for simultaneous quantification in dry-cured ham of an antifungal-peptide Penicillium chrysogenum strain used as protective culture and aflatoxin-producing moulds[J]. Elsevier Ltd, 2014,36(1):257-265.
[80]	Han J, Gao P, Zhao S, et al. iTRAQ-based proteomic analysis of LI-F type peptides produced by Paenibacillus polymyxa JSa-9 mode of action against Bacillus cereus[J]. Elsevier BV, 2017,150:130-140.
[81]	Han J, Wang F, Gao P, et al. Mechanism of action of AMP-jsa9, a LI-F-type antimicrobial peptide produced by Paenibacillus polymyxa JSa-9, against Fusarium moniliforme[J]. Fungal Genetics and Biology, 2017,104:45-55.

来源	前处理	初步分离	纯化	参考文献
蜘蛛	穿刺取血（放在柠檬酸钠缓冲液中,抗凝血）,离心去除血细胞,获得上清液	均质、酸化	反相高效液相色谱	[30]
海洋蜗牛	均质、离心,获得上清液	硫酸铵沉淀、离心、脱盐	反相高效液相色谱	[25]
天南星果	果肉清洗风干、乙醇溶液均质、离心,获得上清液	有机溶剂（丙酮）沉淀	反相高效液相色谱	[35]
蛹虫草	均质,离心,获得上清液	离子交换层析、凝胶过滤层析	快速蛋白质液相色谱	[40]
尼泊尔大红豆	蒸馏水均质,获得上清液	离子交换柱层析、亲和层析	快速蛋白质液相色谱	[37]
苦荞种子	磨碎,磷酸盐缓冲液浸提、离心,获得上清液	硫酸铵沉淀、透析	阴离子交换柱层析、琼脂糖凝胶柱层析、超滤	[34]
豆树种子	Tris-HCl溶液均质,离心,获得上清液	硫酸铵沉淀、透析	离子交换色谱（DEAE-纤维柱）、凝胶过滤色谱	[41]
乳酸菌IS10	发酵液离心,过滤除菌,获得上清液	上清液冷冻干燥	分子筛层析	[64]
芽孢杆菌BH072	离心、过滤除菌,获得滤液	硫酸铵沉淀	凝胶过滤色谱、D201阴离子交换树脂	[52]
内生芽孢杆菌B21	过滤,减压浓缩	有机溶剂（甲醇）萃取,硅胶柱层析	高效液相色谱	[7]
曲霉菌	过滤、离心,获得上清液,耐热性实验	热处理,离心,超滤	反相高效液相色谱	[45]
植物乳杆菌TE10	离心,过滤除菌,获得无细胞上清液	超滤	分子筛层析	[65]
枯草芽孢杆菌CP6	离心,获得上清液,100℃灭菌,冻干	甲醇抽提	离子交换层析、分子筛层析、反相高效液相色谱	[66]
枯草芽孢杆菌1.88株	离心,获得上清液	酸沉、乙醇抽提	反相高效液相色谱	[51]
短小芽孢杆菌HN-10	离心,获得上清液	硫氨酸沉淀、透析	AB-8大孔吸附树脂、Sephadex G-100凝胶、半制备型反相高效液相色谱	[54]
解淀粉芽孢杆菌BW-13	酸碱处理,离心,获得上清液	活性炭吸附	离子交换柱层析	[50]
丝状真菌SIIA-F1108	甲醇沉淀,过滤,获得滤液	大孔树脂吸附	正相硅胶色谱层析、高效液相色谱	[67]

来源	前处理	初步分离	纯化	参考文献
蜘蛛	穿刺取血（放在柠檬酸钠缓冲液中,抗凝血）,离心去除血细胞,获得上清液	均质、酸化	反相高效液相色谱	[30]
海洋蜗牛	均质、离心,获得上清液	硫酸铵沉淀、离心、脱盐	反相高效液相色谱	[25]
天南星果	果肉清洗风干、乙醇溶液均质、离心,获得上清液	有机溶剂（丙酮）沉淀	反相高效液相色谱	[35]
蛹虫草	均质,离心,获得上清液	离子交换层析、凝胶过滤层析	快速蛋白质液相色谱	[40]
尼泊尔大红豆	蒸馏水均质,获得上清液	离子交换柱层析、亲和层析	快速蛋白质液相色谱	[37]
苦荞种子	磨碎,磷酸盐缓冲液浸提、离心,获得上清液	硫酸铵沉淀、透析	阴离子交换柱层析、琼脂糖凝胶柱层析、超滤	[34]
豆树种子	Tris-HCl溶液均质,离心,获得上清液	硫酸铵沉淀、透析	离子交换色谱（DEAE-纤维柱）、凝胶过滤色谱	[41]
乳酸菌IS10	发酵液离心,过滤除菌,获得上清液	上清液冷冻干燥	分子筛层析	[64]
芽孢杆菌BH072	离心、过滤除菌,获得滤液	硫酸铵沉淀	凝胶过滤色谱、D201阴离子交换树脂	[52]
内生芽孢杆菌B21	过滤,减压浓缩	有机溶剂（甲醇）萃取,硅胶柱层析	高效液相色谱	[7]
曲霉菌	过滤、离心,获得上清液,耐热性实验	热处理,离心,超滤	反相高效液相色谱	[45]
植物乳杆菌TE10	离心,过滤除菌,获得无细胞上清液	超滤	分子筛层析	[65]
枯草芽孢杆菌CP6	离心,获得上清液,100℃灭菌,冻干	甲醇抽提	离子交换层析、分子筛层析、反相高效液相色谱	[66]
枯草芽孢杆菌1.88株	离心,获得上清液	酸沉、乙醇抽提	反相高效液相色谱	[51]
短小芽孢杆菌HN-10	离心,获得上清液	硫氨酸沉淀、透析	AB-8大孔吸附树脂、Sephadex G-100凝胶、半制备型反相高效液相色谱	[54]
解淀粉芽孢杆菌BW-13	酸碱处理,离心,获得上清液	活性炭吸附	离子交换柱层析	[50]
丝状真菌SIIA-F1108	甲醇沉淀,过滤,获得滤液	大孔树脂吸附	正相硅胶色谱层析、高效液相色谱	[67]

名称	来源	生物活性	分子质量/Da	参考文献
未命名	七鳃鳗血浆	抗青霉菌、曲霉菌	3 000	[24]
AcICK	蜜蜂体内	抗白僵菌、禾谷镰刀菌	6 600	[27]
LBLP	蜈蚣	抗白色念珠菌、假丝酵母等	2 757.6	[26]
Cm-p1	海洋蜗牛	抗红色毛癣菌、白色念珠菌、假丝酵母菌、灰霉菌、尖孢镰刀菌、黑曲霉等	1 485.26	[25]
Diapausin-1	烟草天蛾幼虫	抗酵母菌、黄色镰刀菌、禾谷镰刀菌等	5 000	[21]
Tn-AFP1	天南星果	抗热带假丝酵母菌	1 230	[35]
Cordymin	蛹虫草	抗玉米小斑病菌、花生球腔菌、立枯丝核菌、白色念珠菌菌丝生长、抗HIV-1逆转录酶和乳腺癌细胞增殖活性	10 906.62	[40]
Eryngin	食用菌杏鲍菇	抗尖孢镰刀菌、花生球腔菌菌丝生长	10 000	[46]
Skh-AMP1	木香叶片	抗曲霉和念珠菌属真菌	2 778.10	[38]
Pleurostrin	平菇	抗尖孢镰刀菌、花生球腔菌、苹果轮纹病菌菌丝生长	7 000	[47]
SmAMP3	鹰嘴豆草	抗腐皮镰孢菌、烟草赤星病菌、根腐离蠕孢、灰霉菌	3 363.9	[39]
未命名	苦荞种子	抗白腐菌、绿色木霉、链格孢霉	3 909	[33]
Campesin	甘蓝菜种子	抗尖孢镰刀菌、花生褐斑病菌,抗HepG2和MCF癌细胞的增殖	9 400	[32]
Ps-AFP1	植物种子	抗尖孢镰刀菌、白色念珠菌、黑曲霉、土曲霉	4 608.20	[68]
鞭毛蛋白	芽孢杆菌BH072	抗黑曲霉、尖孢镰刀菌、腐霉菌、灰霉菌	3 5615	[52]
W10	地衣芽孢杆菌	抗灰霉病菌,具有丝氨酸蛋白酶活性	48 794.16	[43]
AcAFP	曲霉菌	抗尖孢镰刀菌、腐皮镰孢菌、黑曲霉、灰霉菌、番茄早疫病菌	5 773	[45]
PcPAF	海洋桔青霉W1	抗绿色木霉、宛氏拟青霉、长柄链格孢、胶孢炭疽菌	约10 000	[69]
未命名	植物乳杆菌TE10	抗玉米种子黄曲霉MD3生长	664-2 024	[65]
未命名	芽孢杆菌AH-E-1	抗13种植物致病真菌和20种人类致病真菌	500-1 000	[6]
P-1	短小芽孢杆菌HN-10	抗粉红单端孢	1 149.14	[54]
未命名	枯草芽孢杆菌B25	抗尖孢镰刀菌、棒孢霉菌、茄链格孢菌、葡萄孢菌、胶孢炭疽菌、黑曲霉菌	38 708.67	[42]

名称	来源	生物活性	分子质量/Da	参考文献
未命名	七鳃鳗血浆	抗青霉菌、曲霉菌	3 000	[24]
AcICK	蜜蜂体内	抗白僵菌、禾谷镰刀菌	6 600	[27]
LBLP	蜈蚣	抗白色念珠菌、假丝酵母等	2 757.6	[26]
Cm-p1	海洋蜗牛	抗红色毛癣菌、白色念珠菌、假丝酵母菌、灰霉菌、尖孢镰刀菌、黑曲霉等	1 485.26	[25]
Diapausin-1	烟草天蛾幼虫	抗酵母菌、黄色镰刀菌、禾谷镰刀菌等	5 000	[21]
Tn-AFP1	天南星果	抗热带假丝酵母菌	1 230	[35]
Cordymin	蛹虫草	抗玉米小斑病菌、花生球腔菌、立枯丝核菌、白色念珠菌菌丝生长、抗HIV-1逆转录酶和乳腺癌细胞增殖活性	10 906.62	[40]
Eryngin	食用菌杏鲍菇	抗尖孢镰刀菌、花生球腔菌菌丝生长	10 000	[46]
Skh-AMP1	木香叶片	抗曲霉和念珠菌属真菌	2 778.10	[38]
Pleurostrin	平菇	抗尖孢镰刀菌、花生球腔菌、苹果轮纹病菌菌丝生长	7 000	[47]
SmAMP3	鹰嘴豆草	抗腐皮镰孢菌、烟草赤星病菌、根腐离蠕孢、灰霉菌	3 363.9	[39]
未命名	苦荞种子	抗白腐菌、绿色木霉、链格孢霉	3 909	[33]
Campesin	甘蓝菜种子	抗尖孢镰刀菌、花生褐斑病菌,抗HepG2和MCF癌细胞的增殖	9 400	[32]
Ps-AFP1	植物种子	抗尖孢镰刀菌、白色念珠菌、黑曲霉、土曲霉	4 608.20	[68]
鞭毛蛋白	芽孢杆菌BH072	抗黑曲霉、尖孢镰刀菌、腐霉菌、灰霉菌	3 5615	[52]
W10	地衣芽孢杆菌	抗灰霉病菌,具有丝氨酸蛋白酶活性	48 794.16	[43]
AcAFP	曲霉菌	抗尖孢镰刀菌、腐皮镰孢菌、黑曲霉、灰霉菌、番茄早疫病菌	5 773	[45]
PcPAF	海洋桔青霉W1	抗绿色木霉、宛氏拟青霉、长柄链格孢、胶孢炭疽菌	约10 000	[69]
未命名	植物乳杆菌TE10	抗玉米种子黄曲霉MD3生长	664-2 024	[65]
未命名	芽孢杆菌AH-E-1	抗13种植物致病真菌和20种人类致病真菌	500-1 000	[6]
P-1	短小芽孢杆菌HN-10	抗粉红单端孢	1 149.14	[54]
未命名	枯草芽孢杆菌B25	抗尖孢镰刀菌、棒孢霉菌、茄链格孢菌、葡萄孢菌、胶孢炭疽菌、黑曲霉菌	38 708.67	[42]