Polyhydroxybutyrate Production from Mixed Waste Cooking Oil by Cupriavidus necator

doi:10.13560/j.cnki.biotech.bull.1985.2020-1107

Abstract

Abstract:

Waste cooking oil,a kind of hydrocarbon with high carbon content,is one of the main valuable products from the disposal process of kitchen waste. In this study,Cupriavidus necator was employed to synthesize degradable bio-plastics,i.e.,polyhydroxybutyrate（PHB）,with mixed waste cooking oil as sole carbon source. The operational conditions of the PHB synthesis from the waste cooking oil were optimized,and the properties of the synthesized PHB were studied. To lay a foundation for the industrialization of PHB in the future,the PHB yields under different modes of fermentation were explored and compared. The results showed that the maximum yields of the C. necator cell dry weight and PHB were 9.5 g/L and 7.6 g/L under the conditions of 30℃,150 r/min,and pH = 7.0 with amount of 3% waste oil and inoculation addition. Furthermore,the yield of PHB reached 8.25 g/L after fermentation for 96 h by batch culture in a 5 L bio-reactor. The average molecular mass of the synthesized PHB in this study was 30 kD,and the polydispersity index（PDI）was 1.44. The melting temperature of PHB was 175.7℃ and a decomposition temperature of it was 285.5 ^oC,which may meet the requirements of thermoplastic materials.

Key words: waste cooking oil, bio-plastics, polyhydroxybutyrate, Cupriavidus necator

PAN Lan-jia, LI Jie, LIN Qing-huai, WANG Yin. Polyhydroxybutyrate Production from Mixed Waste Cooking Oil by Cupriavidus necator[J]. Biotechnology Bulletin, 2021, 37(4): 127-136.

Figures/Tables 12

Table 1 Fatty acid content of the waste cooking oil

脂肪酸Fatty acid	含量Content/%
十六烷酸Palmitic acid（C16：0）	14.887
十八烷酸Stearic acid（C18：0）	4.097
十八碳烯酸Octadecenoic acid（C18：1）	31.000
亚麻酸Linolenic acid（C18：3）	28.380
亚油酸Linoleic acid（C18：2）	18.086
其他Others	3.550

Fig. 1 Identification of methylated products of PHA by gas chromatography-mass spectrometry

Fig. 2 Effect of temperature on the cell dry weight of strain C. necator and PHB synthesis Different capital letters represent significant difference (P < 0.05); different lowercase letters represent the percentage of PHB has significant difference (P < 0.05), the same below

Fig.3 Effect of pH on the cell dry weight of strain C. necator and PHB synthesis

Fig.4 Effect of rotation speed on the cell dry weight of strain C. necator and PHB synthesis

Fig.5 Effect of waste cooking oil amount on the cell dry weight of strain C. necator and PHB synthesis

Fig.6 Effect of inoculum amount on the cell dry weight of strain C. necator and PHB synthesis

Fig.7 Effect of culture time on the cell dry weight of strain C. necator and PHB synthesis

Table 2 Bacterial cell and PHB production under different fermentation modes

模式 Mode	菌体干重 Cell dry weight/（g·L^-1）	PHB含量 PHB content/wt%	PHB/（g·L^-1）
M1	10.75	76.75	8.25
M2	7.62	86.88	6.62
M3	6.79	82.06	5.57

Table 3 Experiment results of fermentation model 1

循环 Circulation	菌体干重 Cell dry weight/（g·L^-1）	PHB含量 PHB content /wt%	PHB/（g·L^-1）
1	10.75	76.75	8.25
2	12.38	76.77	9.50
3	11.10	76.22	8.46
4	12.12	77.36	9.38

Table 4 Molecular weight distribution and thermodynamic properties of PHB synthesized by bacteria

菌种Strain	碳源Carbon source	M_n/kD	M_w/kD	PDI	熔化温度 Melting temperature/℃	分解温度Decomposition temperature/℃	参考文献Reference
B. thailandensis	餐厨废油 Waste cooking oil	179	511	2.86	166.4	279.3	[17]
C. necator DSM428	餐厨废油 Waste cooking oil	110	170	1.5	168.6	-	[19]
C. necator DSM428	咖啡渣油 Coffee residue oil	-	234	1.2	172.3	-	[20]
C. necator（CGMCC 1.7092）	混合餐厨废油 Mixed waste cooking oil	20	30	1.44	175.7	285.5	本研究 This study

Fig.8 Differential scanning calorimetry analysis (A) and thermogravimetric analysis (B)

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