Study on Antibacterial Activity and Mechanism of Cordycepin

doi:10.13560/j.cnki.biotech.bull.1985.2020-1169

Abstract

Abstract:

Cordycepin is the main active component of Cordyceps militaris and other food and health products,the purpose of the study is to further evaluate and analyze the main antibacterial activity and mechanism of cordycepin. The bacteriostatic spectrum and effect of cordycepin on six bacteria were determined by minimum inhibitory concentration,bacteriostatic zone test and electron microscopic observation,and the growth curve,intracellular ultraviolet absorption substances leakage and morphological changes of the tested bacteria were further determined to analyze the mechanism of cordycepin inhibiting cell growth and destroying cell structure. The results showed that cordycepin had obvious bacteriostatic effect on three G⁺bacteria in the range of solubility,but had no obvious bacteriostatic effect on three G^-bacteria. Growth assay and electron microscopic observation showed that the morphology of G⁺ bacteria was seriously damaged after cordycepin treatment,the intracellular ultraviolet absorption substances were leaked out,and the bacterial growth process was blocked. While the surface of some tested G^-bacteria was slightly damaged and accompanied by a small amount of content leaked,but the bacterial growth process was not affected. Cordycepin presents certain selectivity in bacteriostasis,and it is easier to destroy the wall membrane system of the tested G⁺ bacteria and play a bacteriostatic role,but has no significant effect on the overall growth process of the tested G^-bacteria.

Key words: cordycepin, minimum inhibitory concentration, inhibitory zone, growth curve, bacterial morphology, antibacterial mechanism

GAO Su, MA Jie-xin, LIU Jing-ju, ZHAO Guo-zhu. Study on Antibacterial Activity and Mechanism of Cordycepin[J]. Biotechnology Bulletin, 2021, 37(4): 137-144.

Figures/Tables 7

Table1 Determination of cordycepin MIC

供试菌株 Tested strains	虫草素浓度 Concentration of cordycepin/（mg·mL^-1）
供试菌株 Tested strains	0	0.625	1.25	2.5	5	10
B. subtilis BS-1	+++	++	+	-	-	-
B. thuringiensis BT-1	+++	+++	++	+	-	-
S. aureus SA-1	+++	+++	++	+	-	-
E. coli EC-1	+++	+++	+++	+++	+++	+
S. enteritidis SE-1	+++	+++	+++	+++	+++	+
P. aeruginosa PA-1	+++	+++	+++	+++	+++	++

Fig.1 DIZ of cordycepin against six tested strains A is the control well. B, C and D are 2.5 mg/mL, 5 mg/mL and 10 mg/mL. respectively cordycepin group

Table 2 Determination of cordycepin DIZ

供试菌株 Tested strains	DIZ/mm
供试菌株 Tested strains	2.5 mg/mL 虫草素cordycepin	5 mg/mL 虫草素 cordycepin	10 mg/mL 虫草素 cordycepin
B. subtilis BS-1	3.6 ± 0.17^d	5.2 ± 0.16^b	8.75 ± 0.21^a
B. thuringiensis BT-1	0	3.52 ± 0.23^d	5.25 ± 0.15^b
S. aureus SA-1	0	2.97 ± 0.20^e	4.15 ± 0.31^c
E. coli EC-1	0	0	0
S. enterica SE-1	0	0	0
P. aeruginosa PA-1	0	0	0

Fig.2 Effects of cordycepin on the growth curves of six tested strains

Fig.3 Effects of cordycepin on the leakage of intracellular ultraviolet absorbents of six tested strains

Fig.4 Morphology of tested G+ bacteria under field emission scanning electron microscope Capital letters refer to each experimental group, and lowercase letters refer to each control group. A, a: B.Subtilis BS-1. B, b: B. thuringiensis BT-1. C, c: S.aureus SA-1. Magnification: 8000×. Scale: 5 μm

Fig.5 Morphology of tested G- bacteria under field emission scanning electron microscope Capital letters refer to each experimental group, and lowercase letters refer to each control group. A, a: E. coli EC-1. B, B: S. enteritidis SE - 1. C, c: P.aeruginosa PA-1.Magnification: 8000×. Scale: 5 μm

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