Effects of Biofumigation on Root-knot Nematodes and Soil Nematode Community

doi:10.13560/j.cnki.biotech.bull.1985.2021-0670

Abstract

Abstract:

Root-knot nematodes are important pathogens threatening agricultural production. The control effects of cabbage biofumigation on tomato root-knot nematodes in the field were studied,the soil nematodes were isolated by the suspended centrifugation method and the influence of biofumigation on the soil nematode community was evaluated. The results showed that cabbage biofumigation reduced the damage of root-knot nematode to tomato. The control efficacy of biofumigation was 58.6% and 45.8% respectively at 60 and 90 d post transplantation,which was equivalent to that of the chemical pesticide fosthiazate. In addition,biofumigation resulted in the increase of the number of bacterivores nematodes and omnivore predator nematodes,and reduction of the number of fungivores nematodes and parasitic nematodes. Moreover biofumigation increased the maturity index（MI）of soil free-living nematodes,reduced the plant parasite index（PPI）and PPI/MI. It is indicated that cabbage biofumigation presents some inhibitory effect on plant parasitic nematodes,reduces the number and intensity of soil interference,and plays a positive role in the soil ecological environment. The research results show that cabbage biofumigation may effectively prevent and control vegetable root-knot nematodes,which is an environmentally friendly green prevention and control technology.

Key words: root-knot nematodes, biofumigation, nematode community, cabbage

JIN Na, WANG Xue-yan, LIU Qian, PENG De-liang, PENG Huan, JIAN Heng. Effects of Biofumigation on Root-knot Nematodes and Soil Nematode Community[J]. Biotechnology Bulletin, 2021, 37(7): 156-163.

Figures/Tables 7

References 20

[1]	Vanegas-Villa DM, Navarro-Álzate RA, Afanador-Kafuri L, et al. Effect of interaction between Phytophthora nicotianae and Meloidogyne spp. on the productivity and quality of tobacco plants(Nicotiana tabacum)[J]. Nematology, 2020, 22(10):1179-1192. doi: 10.1163/15685411-bja10021 URL
[2]	Keter SJ, Nyalala S, Ogweno J. Biofumigation efficacy of spider plant(Cleome gynandra L.)accessions on nematode control in tuberose(Polianthes tuberosa L.)[J]. NASS J Agric Sci, 2020, 2(2):15-23.
[3]	金娜. 红灰链霉菌HDZ-9-47生产技术及对土壤生物群落的影响研究[D]. 北京:中国农业大学, 2016.
	Jin N. Production technique and effects on soil biological community of Streptomyces rubrogriseus HDZ-9-47[D]. Beijing:China Agricultural University, 2016.
[4]	卢志军. 蔬菜根结线虫病生物熏蒸控制作用研究[D]. 北京:中国农业大学, 2016.
	Lu ZJ. Control effeciency of biological fumigation against vegetable root-knot nematodes[D]. Beijing:China Agricultural University, 2016.
[5]	Jin N, Xue H, Li WJ, et al. Field evaluation of Streptomyces rubrogriseus HDZ-9-47 for biocontrol of Meloidogyne incognita on tomato[J]. J Integr Agric, 2017, 16(6):1347-1357. doi: 10.1016/S2095-3119(16)61553-8 URL
[6]	王纪磊, 李霜, 史庆华. 花椒籽(饼)熏蒸对温室根结线虫防效及番茄生长发育的影响[J]. 山东农业科学, 2020, 52(1):120-125.
	Wang JL, Li S, Shi QH. Effect of Zanthoxylum bungeanum seeds(cake)fumigation on control of root-knot nematode as well as growth and development of tomato in greenhouse[J]. Shandong Agric Sci, 2020, 52(1):120-125.
[7]	Bridge J, Page SLJ. Estimation of root-knot nematode infestation levels on roots using a rating chart[J]. Trop Pest Manag, 1980, 26(3):296-298. doi: 10.1080/09670878009414416 URL
[8]	刘维志. 植物线虫志[M]. 北京: 中国农业出版社, 2004.
	Liu WZ. Description of the species of plant parasitic nematodes[M]. Beijing: Chinese Agriculture Press, 2004.
[9]	冯志新. 植物线虫学[M]. 北京: 中国农业出版社, 2001.
	Feng ZX. Plant nematology[M]. Beijing: China Agriculture Press, 2001.
[10]	尹文英. 中国土壤动物检索图鉴[M]. 北京: 科学出版社, 1998.
	Yin WY. Pictorical keys to soil animals of China[M]. Beijing: Science Press, 1998.
[11]	Guo XH, Endler A, Poll C, et al. Independent effects of warming and altered precipitation pattern on nematode community structure in an arable field[J]. Agric Ecosyst Environ, 2021, 316:107467. doi: 10.1016/j.agee.2021.107467 URL
[12]	Bongers T. The maturity index:an ecological measure of environmental disturbance based on nematode species composition[J]. Oecologia, 1990, 83(1):14-19. doi: 10.1007/BF00324627 pmid: 28313236
[13]	Rubayet MT, Bhuiyan M, Jannat R, et al. Effect of biofumigation and soil solarization on stem canker and black scurf diseases of potato(Solanum tuberosum L.)caused by Rhizoctoniasolani PR2 isolate[J]. Advances in Agricultural Sciences, 2018, 6(3):33-38.
[14]	李世东, 李明社, 缪作清, 等. 生物熏蒸用于治理蔬菜根结线虫病的研究[J]. 植物保护, 2007, 33(4):68-71.
	Li SD, Li MS, Miao ZQ, et al. Soil biofumigation for management of vegetable root-knot nematode disease[J]. Plant Prot, 2007, 33(4):68-71.
[15]	曹素芳. 生物熏蒸和温湿度对南方根结线虫的影响及化学防治的研究[D]. 兰州:甘肃农业大学, 2009.
	Cao SF. Effect of biofumigation and temperature and moisture on the survival of Meloidogyne incongnita and its chemical control[D]. Lanzhou:Gansu Agricultural University, 2009.
[16]	Jin N, Lu X, Wang X, et al. The effect of combined application of Streptomyces rubrogriseus HDZ-9-47 with soil biofumigation on soil microbial and nematode communities[J]. Sci Rep, 2019, 9(1):16886. doi: 10.1038/s41598-019-52941-9 pmid: 31729417
[17]	陈立杰, 段玉玺, 梁文举, 等. 涕灭威对大豆田土壤线虫生物多样性的影响[J]. 大豆科学, 2006, 25(2):164-169.
	Chen LJ, Duan YX, Liang WJ, et al. Effects of aldicarb on community structure and bio-diversity of soil nematodes in soyhean field[J]. Soybean Sci, 2006, 25(2):164-169.
[18]	陈立杰, 梁文举, 段玉玺, 等. 施用生防颗粒剂对大豆田土壤线虫群落结构和生物多样性的影响[J]. 大豆科学, 2003, 22(4):251-256.
	Chen LJ, Liang WJ, Duan YX, et al. Effects of bio-nematicide on community structure and bio-diversity of soil nematodes in soybean field[J]. Soybean Sci, 2003, 22(4):251-256.
[19]	梁文举, 葛亭魁, 段玉玺. 土壤健康及土壤动物生物指示的研究与应用[J]. 沈阳农业大学学报, 2001, 32(1):70-72.
	Liang WJ, Ge TK, Duan YX. Bioindication of soil fauna to soil health[J]. J Shenyang Agric Univ, 2001, 32(1):70-72.
[20]	陈瑶. 不同复种方式对北京保护地土栖线虫群落结构的影响[D]. 雅安:四川农业大学, 2006.
	Chen Y. Effect of different multiple cropping on soil nematode communities in Beijing greenhouse[D]. Yaan:Sichuan Agricultural University, 2006.

处理 Treatment	60 d			90 d
处理 Treatment	根结级数 Root knot series	病情指数Disease index	防治效率 Control efficiency/%	根结级数 Root knot series	病情指数Disease index	防治效率 Control efficiency/%
CK	5.80±0.45a	58.00		6.92±0.79a	69.17	—
噻唑膦Fosthiazate	2.20±0.84b	22.00	62.1	2.25±1.36b	22.50	67.5
生物熏蒸Biofumigation	2.40±1.14b	24.00	58.6	3.75±0.97b	37.50	45.8
生物熏蒸+HDZ-9-47 Biofumigation +HDZ-9-47	2.00±1.00b	20.00	65.5	3.11±1.17b	31.11	55.0

处理 Treatment	60 d			90 d
处理 Treatment	根结级数 Root knot series	病情指数Disease index	防治效率 Control efficiency/%	根结级数 Root knot series	病情指数Disease index	防治效率 Control efficiency/%
CK	5.80±0.45a	58.00		6.92±0.79a	69.17	—
噻唑膦Fosthiazate	2.20±0.84b	22.00	62.1	2.25±1.36b	22.50	67.5
生物熏蒸Biofumigation	2.40±1.14b	24.00	58.6	3.75±0.97b	37.50	45.8
生物熏蒸+HDZ-9-47 Biofumigation +HDZ-9-47	2.00±1.00b	20.00	65.5	3.11±1.17b	31.11	55.0

营养类群Trophic group	目Order	科Family	属Genus	cp值cp value
植物寄生类	垫刃目Tylenchida	异皮科Heteroderidae	根结线虫Meloidogyne	3
Plant parasites			茎线虫Ditylenchus	2
		短体科Pratylenchidae	短体属Pratylenchus	2
			丝尾线虫属Filenchus	2
			小环属Criconemella	3
		垫刃科Tylenchidae	垫刃属Tylenchus	2
食真菌类	滑刃目Aphelenchida	滑刃科Aphelenchoididae	滑刃属Aphelenchoides	2
Fungivorcs		真滑刃科Aphelenchidae	真滑刃属Aphelenchus	2
			长尾线虫属Seinura	2
食细菌类	小杆目Rhabditida	头叶科Cephalobidae	丽突属Acrobeles	2
Bacterivores			拟丽突属Arcobeloides	2
			鹿角唇属Cervidellus	2
		小杆科Rhabditidae	小杆属Rhabditis	1
			广杆属Caenorhabditis	1
			原杆属Protorhabditis	1
		双胃科Diplogasteridae	双胃属Diplogaster	1
杂食/捕食类	矛线目Dorylaimida	长针科Longidoridae	剑线虫Xiphinema	5
Predator/Omnivores		矛线科Dorylaimidae	矛线属Dorylaimus	4
		未知 Unknown	未知 Unknown	4
	三矛目Triplochida	未知 Unknown	未知 Unknown	3
	嘴刺目Enoplida	伊龙科Ironidae	伊龙属Ironus	5
			未知 Unknown	5

营养类群Trophic group	目Order	科Family	属Genus	cp值cp value
植物寄生类	垫刃目Tylenchida	异皮科Heteroderidae	根结线虫Meloidogyne	3
Plant parasites			茎线虫Ditylenchus	2
		短体科Pratylenchidae	短体属Pratylenchus	2
			丝尾线虫属Filenchus	2
			小环属Criconemella	3
		垫刃科Tylenchidae	垫刃属Tylenchus	2
食真菌类	滑刃目Aphelenchida	滑刃科Aphelenchoididae	滑刃属Aphelenchoides	2
Fungivorcs		真滑刃科Aphelenchidae	真滑刃属Aphelenchus	2
			长尾线虫属Seinura	2
食细菌类	小杆目Rhabditida	头叶科Cephalobidae	丽突属Acrobeles	2
Bacterivores			拟丽突属Arcobeloides	2
			鹿角唇属Cervidellus	2
		小杆科Rhabditidae	小杆属Rhabditis	1
			广杆属Caenorhabditis	1
			原杆属Protorhabditis	1
		双胃科Diplogasteridae	双胃属Diplogaster	1
杂食/捕食类	矛线目Dorylaimida	长针科Longidoridae	剑线虫Xiphinema	5
Predator/Omnivores		矛线科Dorylaimidae	矛线属Dorylaimus	4
		未知 Unknown	未知 Unknown	4
	三矛目Triplochida	未知 Unknown	未知 Unknown	3
	嘴刺目Enoplida	伊龙科Ironidae	伊龙属Ironus	5
			未知 Unknown	5