Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (7): 164-174.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0408
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SHU Jie1,2(), ZHANG Ren-jun1,2, LIANG Ying-chong1,2, CHEN Ya-qiong3(), ZHANG Juan4, GUO Jian5, CHEN Sui-yun2,6()
Received:
2021-04-01
Online:
2021-07-26
Published:
2021-08-13
Contact:
CHEN Ya-qiong,CHEN Sui-yun
E-mail:1043317148@qq.com;312547613@qq.com;976809218@qq.com
SHU Jie, ZHANG Ren-jun, LIANG Ying-chong, CHEN Ya-qiong, ZHANG Juan, GUO Jian, CHEN Sui-yun. Control of Root-knot Nematode Disease by Compounding Biological Agents from Plant and Microorganisms[J]. Biotechnology Bulletin, 2021, 37(7): 164-174.
Fig. 5 Virulence determination of nematodes under microscope ① LB control;② SJ5 fermentation filtrate;③ SJ17 fermentation filtrate;④ SJ19 fermentation filtrate;⑤ Sophora azadirachtin dilution(1 000 times). The bent body of the nematode indicates that it is in a living state,and the arc or straight shape indicates that it is dead or stiff and dying
Fig. 6 Differences in virulence test of different treatment groups * indicates a significant difference level 0.01<P<0.05,** indicates P <0.01,and n.s. indicates no significant difference
Fig. 7 Compatible test results A:SJ5 is used for coating,and SJ17 and SJ19 are dot-coated on the plate.B:SJ17 is used for coating the plate,and SJ5 and SJ19 are dot-coated on the plate respectively
Fig. 8 Effects of different treatments on tomato root system(45 d) The points where the red arrows in the picture point to represents root knots on tomato roots,that is,nodules formed by root-knot nematode parasites
Fig. 9 Control effects of different treatments on tomato root-knot nematode disease(45 d) Different letters refer to significant differences(P < 0.05)
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