Fig. 1 Diagram of fluorescent labeled cytoskeleton vectors A: The diagram of microfilament labeling vector through Lifeact-EGFP; LA: coding sequence of Lifeact; PgpdA: promoter of glyceraldehyde-3-phosphate dehydrogenase in Aspergillus nidulans; TtrpC: terminator of tryptophan synthesis gene in Aspergillus nidulans; HPT：Hygromycin phosphate transferase resistance gene. B: The diagram of microtubule labeling vector through MBD-EGFP; MBD：microtubule binding domain. C: The diagram of inserting EGFP coding sequence behind gene CgTUB1 through homologous recombination; WT: wild type; Mutant: mutant strain; Flanking region: downstream of gene CgTUB1

Fig. 2 PCR diagnosis of cytoskeleton labeling recombinant strains A: PCR diagnosis of microfilament labeling strains by Lifeact-EGFP. B：PCR diagnosis of microtubule labeling strains by MBD-EGFP. C: PCR diagnosis of the upstream recombinant fragment of CgTUB1-EGFP labeling strains. D: PCR diagnosis of the downstream recombinant fragment of CgTUB1-EGFP labeling strains. M: DNA marker DM5000

Fig. 3 Cytoskeleton structure in conidiophores of fluorescent labeled recombinant strains CK: Control strain expressing EGFP; Lifeact-EGFP: microfilament labeled recombinant strain; CgTUB1-EGFP: microtubule labeled recombinant strain; Z Projection：stacked image of Z-series slices; scale bar：5 μm. The same below

Fig. 4 Cytoskeleton structure in the hypha of fluorescent labeled recombinant strains

Fig. 5 Clony diameters and conidiations of WT and mutant strains A: Clony diameter of WT and mutant strains on PDA media; B: conidiation of WT and mutant strains on PDB liquid media; WT: wild type strain

Fig. 6 Virulence assay on rubber tree leaves and lesion diameter after inoculation with WT and recom-binant strains A: Disease symptoms of rubber tree leaves at day 3 after inoculation (dpi); B: Mean lesion diameters of rubber tree leaves at day 2 and 3 (dpi).