Fig. 1 Homologous recombination principle of the gene CgaswA Sur is the chlorimuron-ethyl resistance gene,and black triangles indicate the primers used to verify gene-knockout mutants

Fig. 2 Protein domain and phylogenetic tree of CgAswA protein A：Protein domain analysis of CgAswA. B：Phylogenetic analysis of CgAswA. The sequence ID is in parentheses;the numbers on the horizontal lines represent evolutionary distances

Fig. 3 Verification of CgaswA gene knockout and com-plementation A：PCR results of CgaswAF / CgaswAR;B：PCR results of CgaswAUU / PI;C：PCR results of PI1 / CgaswADD. M：DL2 000 DNA marker;1：wild type;2：ΔCgaswA-3;3：ΔCgaswA-8;4：ΔCgaswA-11;5：ΔCgaswA/aswA

Fig. 4 Comparison of strains’ growth on four media A：Growth of strains on four media. B：Statistical analysis of colony diameter. a,b：Significant level,P<0.05

Fig. 5 Sporulation,germination and appressorium formation of wild type,ΔCgaswA and ΔCgaswA/aswA A：Statistical results of conidia yield. B：Statistical results of conidia germination rate. C：Statistical results of appressorium formation rate. D：Conidial germination and appressorium formation at 4 h,8 h and 12 h;co：conidium,gt：germ tube,ap：appressorium;a,b,c,d：significant level,P<0.05. Scale bars = 20 µm

Fig. 6 Onion epidermis infection and cellophane penetration assays A：Appressorium infection assay of different strains on onion epidermis. B：Cellophane penetration assay of conidia suspensions. C：Cellophane penetration assay of mycelial plugs;co：conidium,ap：appressorium,ih：invasive hyphae;a,b,c,d：significant level,P<0.05;scale bars = 20 µm

Fig. 7 Pathogenicity analyses of wild type,ΔCgaswA and ΔCgaswA/aswA A：Symptoms on rubber leaves at 4 d post-inoculation using mycelial plugs and statistical analyses of the diameters of disease spots. B：Symptomss on rubber leaves at 5 d post-inoculation using conidial suspensions and statistical analyses of the diameters of disease spots. 1：CK;2：WT;3：ΔCgaswA/ aswA;4：ΔCgaswA-3;5：ΔCgaswA-8;6：ΔCgaswA-11;a,b,c,d：significant level,P<0.05