Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (12): 50-59.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0844
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XIONG Xue1(), LI Peng1, ZHANG Gui-he2, XIANG Zhun1(), TAO Wen-Guang2, ZHOU Guang-yan2, HE Yao-wei1
Received:
2021-05-25
Online:
2021-12-26
Published:
2022-01-19
Contact:
XIANG Zhun
E-mail:604051569@qq.com;xiangzhun@163.com
XIONG Xue, LI Peng, ZHANG Gui-he, XIANG Zhun, TAO Wen-Guang, ZHOU Guang-yan, HE Yao-wei. Effects of Different Cultivation Substrates on the Laccase Activities of Lentinula edodes During Liquid Fermentation[J]. Biotechnology Bulletin, 2021, 37(12): 50-59.
菌株Strain | 马桑香菇 Masang L. edodes | 香菇808 L. edodes 808 | 庆科R20 Qingke R20 |
---|---|---|---|
马桑香菇 Masang L. edodes | - | ||
香菇808 L. edodes 808 | + | - | |
庆科R20 Qingke R20 | + | - | - |
Table 1 Antagonistic reaction of 3 kinds of L. edodes strain
菌株Strain | 马桑香菇 Masang L. edodes | 香菇808 L. edodes 808 | 庆科R20 Qingke R20 |
---|---|---|---|
马桑香菇 Masang L. edodes | - | ||
香菇808 L. edodes 808 | + | - | |
庆科R20 Qingke R20 | + | - | - |
Fig. 1 Antagonistic reaction of 3 kinds of L. edodes strain A:Masang L. edodes×Masang L. edodes. B:L. edodes 808×L. edodes 808. C:Qingke R20×Qingke R20. D:Qingke R20(left)×Masang L. edodes(underside)×L. edodes 808(right)
菌株 Strain | 温度 Temperature/℃ | 萌发时间Germination time/h | 菌落长势Colony growth | 菌落描述 Colony description | 日均生长速度 Average daily growth rate/(cm·d-1) | 差异显著性 Significant difference (P<0.05) |
---|---|---|---|---|---|---|
马桑香菇 Masang L. edodes | 10 | 48 | ++ | 绒毛状白色圆形菌落,边缘整齐 | 0.11 | e |
15 | 48 | +++ | 绒毛状白色圆形菌落,边缘整齐 | 0.34 | d | |
20 | 48 | ++++ | 绒毛状白色圆形菌落,边缘整齐 | 0.49 | b | |
25 | 48 | ++++ | 绒毛状白色圆形菌落,边缘整齐 | 0.89 | a | |
30 | 48 | +++ | 绒毛状白色圆形菌落,边缘整齐 | 0.41 | c | |
香菇808 L. edodes 808 | 10 | 48 | + | 绒毛状白色圆形菌落,边缘整齐 | 0.15 | d |
15 | 48 | ++ | 绒毛状白色圆形菌落,边缘整齐 | 0.28 | c | |
20 | 48 | +++ | 绒毛状白色圆形菌落,边缘整齐 | 0.44 | b | |
25 | 48 | ++++ | 绒毛状白色圆形菌落,边缘整齐 | 0.85 | a | |
30 | 48 | ++++ | 绒毛状白色圆形菌落,边缘整齐 | 0.31 | c | |
庆科R20 Qingke R20 | 10 | 48 | ++ | 绒毛状白色圆形菌落,边缘整齐 | 0.10 | e |
15 | 48 | +++ | 绒毛状白色圆形菌落,边缘整齐 | 0.29 | c | |
20 | 48 | +++ | 绒毛状白色圆形菌落,边缘整齐 | 0.50 | b | |
25 | 48 | ++++ | 绒毛状白色圆形菌落,边缘整齐 | 0.89 | a | |
30 | 48 | ++ | 绒毛状白色圆形菌落,边缘较整齐 | 0.25 | d |
Table 2 Effects of different temperature conditions on the growths of 3 kinds of L. edodes strain
菌株 Strain | 温度 Temperature/℃ | 萌发时间Germination time/h | 菌落长势Colony growth | 菌落描述 Colony description | 日均生长速度 Average daily growth rate/(cm·d-1) | 差异显著性 Significant difference (P<0.05) |
---|---|---|---|---|---|---|
马桑香菇 Masang L. edodes | 10 | 48 | ++ | 绒毛状白色圆形菌落,边缘整齐 | 0.11 | e |
15 | 48 | +++ | 绒毛状白色圆形菌落,边缘整齐 | 0.34 | d | |
20 | 48 | ++++ | 绒毛状白色圆形菌落,边缘整齐 | 0.49 | b | |
25 | 48 | ++++ | 绒毛状白色圆形菌落,边缘整齐 | 0.89 | a | |
30 | 48 | +++ | 绒毛状白色圆形菌落,边缘整齐 | 0.41 | c | |
香菇808 L. edodes 808 | 10 | 48 | + | 绒毛状白色圆形菌落,边缘整齐 | 0.15 | d |
15 | 48 | ++ | 绒毛状白色圆形菌落,边缘整齐 | 0.28 | c | |
20 | 48 | +++ | 绒毛状白色圆形菌落,边缘整齐 | 0.44 | b | |
25 | 48 | ++++ | 绒毛状白色圆形菌落,边缘整齐 | 0.85 | a | |
30 | 48 | ++++ | 绒毛状白色圆形菌落,边缘整齐 | 0.31 | c | |
庆科R20 Qingke R20 | 10 | 48 | ++ | 绒毛状白色圆形菌落,边缘整齐 | 0.10 | e |
15 | 48 | +++ | 绒毛状白色圆形菌落,边缘整齐 | 0.29 | c | |
20 | 48 | +++ | 绒毛状白色圆形菌落,边缘整齐 | 0.50 | b | |
25 | 48 | ++++ | 绒毛状白色圆形菌落,边缘整齐 | 0.89 | a | |
30 | 48 | ++ | 绒毛状白色圆形菌落,边缘较整齐 | 0.25 | d |
培养时间 Incubation period/d | 漆酶活性Laccase activity/(U·L-1) | |||
---|---|---|---|---|
菌株 Strain | 培养基 Substrate | 菌株×培养基 Strain × Substrate | ||
2 | 65.718*** | 23.380*** | 6.887*** | |
3 | 38.515*** | 26.703*** | 5.890*** | |
4 | 23.114*** | 30.396*** | 21.908*** | |
5 | 40.804*** | 31.882*** | 31.989*** | |
6 | 59.004*** | 24.854*** | 41.526*** | |
7 | 212.19*** | 79.513*** | 80.377*** | |
8 | 353.451*** | 133.537*** | 111.936*** | |
9 | 302.412*** | 96.631*** | 67.755*** | |
10 | 537.164*** | 143.962*** | 115.817*** | |
11 | 371.454*** | 164.037*** | 91.993*** | |
12 | 93.704*** | 45.079*** | 34.453*** |
Table 3 Effects of 4 cultivation substrates on the laccase activities of different L. edodes
培养时间 Incubation period/d | 漆酶活性Laccase activity/(U·L-1) | |||
---|---|---|---|---|
菌株 Strain | 培养基 Substrate | 菌株×培养基 Strain × Substrate | ||
2 | 65.718*** | 23.380*** | 6.887*** | |
3 | 38.515*** | 26.703*** | 5.890*** | |
4 | 23.114*** | 30.396*** | 21.908*** | |
5 | 40.804*** | 31.882*** | 31.989*** | |
6 | 59.004*** | 24.854*** | 41.526*** | |
7 | 212.19*** | 79.513*** | 80.377*** | |
8 | 353.451*** | 133.537*** | 111.936*** | |
9 | 302.412*** | 96.631*** | 67.755*** | |
10 | 537.164*** | 143.962*** | 115.817*** | |
11 | 371.454*** | 164.037*** | 91.993*** | |
12 | 93.704*** | 45.079*** | 34.453*** |
Fig.5 Comparison of the laccase production activities of 3 kinds of L. edodes strains in liquid fermentation on different induction media A:BM+MS:Basic medium with C. palensis sawdust. B:BM+QG:Basic medium with Q. acutissima sawdust. C:BM+YMX:Basic medium with corncob. D:BM+YMX:Basic medium with wheat bran
Fig.6 Effects of different cultivation substrates on laccase production activities of different L. edodes strains A:Marsang L. edodes. B:L. edodes 808. C:Qingke R20
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