Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (12): 82-90.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0179
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TANG Ying(), HUANG Jia, DENG Zhan-rui, YANG Xiao-nan
Received:
2021-02-09
Online:
2021-12-26
Published:
2022-01-19
TANG Ying, HUANG Jia, DENG Zhan-rui, YANG Xiao-nan. Product Analysis of Degrading Aflatoxin B1 by a Strain Bacillus subtilis[J]. Biotechnology Bulletin, 2021, 37(12): 82-90.
培养时间Incubation time | 组别 Group | Viable Cell Density (×106 cells/mL)±RSD | Average date for double tine±RSD/h | Average date for genration number | P |
---|---|---|---|---|---|
0 d | 参照组 | 0.010±0.021 | / | / | / |
试验组 | 0.010±0.016 | / | / | / | |
对照组 | 0.010±0.020 | / | / | / | |
1 d | 参照组 | 0.029±013 | 21.609±0.74 | 1.362 | >0.05 |
试验组 | 0.022±0.023 | 21.920±0.98 | 1.293 | >0.05 | |
对照组 | 0.013±0.031 | 45.270±1.20 | 0.821 | <0.05 | |
2 d | 参照组 | 0.063±0.016 | 22.937±0.72 | 2.654 | >0.05 |
试验组 | 0.053±0.022 | 22.603±0.46 | 2.437 | >0.05 | |
对照组 | 0.008±0.035 | 57.558±1.03 | 0.629 | <0.05 | |
3 d | 参照组 | 0.131±0.031 | 21.193±0.59 | 3.815 | >0.05 |
试验组 | 0.116±0.027 | 22.462±0.91 | 3.623 | >0.05 | |
对照组 | 0.003±0.010 | 68.361±1.31 | 0.231 | <0.05 |
Table 1 Effects of degraded products on the growth of L-02 cells
培养时间Incubation time | 组别 Group | Viable Cell Density (×106 cells/mL)±RSD | Average date for double tine±RSD/h | Average date for genration number | P |
---|---|---|---|---|---|
0 d | 参照组 | 0.010±0.021 | / | / | / |
试验组 | 0.010±0.016 | / | / | / | |
对照组 | 0.010±0.020 | / | / | / | |
1 d | 参照组 | 0.029±013 | 21.609±0.74 | 1.362 | >0.05 |
试验组 | 0.022±0.023 | 21.920±0.98 | 1.293 | >0.05 | |
对照组 | 0.013±0.031 | 45.270±1.20 | 0.821 | <0.05 | |
2 d | 参照组 | 0.063±0.016 | 22.937±0.72 | 2.654 | >0.05 |
试验组 | 0.053±0.022 | 22.603±0.46 | 2.437 | >0.05 | |
对照组 | 0.008±0.035 | 57.558±1.03 | 0.629 | <0.05 | |
3 d | 参照组 | 0.131±0.031 | 21.193±0.59 | 3.815 | >0.05 |
试验组 | 0.116±0.027 | 22.462±0.91 | 3.623 | >0.05 | |
对照组 | 0.003±0.010 | 68.361±1.31 | 0.231 | <0.05 |
[1] | 罗自生, 秦雨, 等. 黄曲霉毒素的生物合成、代谢和毒性研究进展[J]. 食品科学, 2015, 36(3):250-257. |
Luo ZS, Qin Y, et al. Recent progress in the biosynjournal, metabolism and toxicity of aflatoxins[J]. Food Sci, 2015, 36(3):250-257. | |
[2] | 乔宏兴, 姜亚乐, 等. 黄曲霉毒素的危害及其脱毒方法研究进展[J]. 动物医学进展, 2017, 38(1):89-93. |
Qiao HX, Jiang YL, et al. Progress on harm and detoxification methods of aflatoxin[J]. Prog Vet Med, 2017, 38(1):89-93. | |
[3] | 赵萌, 高婧, 等. 黄曲霉毒素B1的分子致毒机理及其微生物脱毒研究进展[J]. 食品科学, 2019, 40(11):235-245. |
Zhao M, Gao J, et al. Recent progress in research on toxicity mechanism and microbial detoxification of aflatoxin B1[J]. Food Sci, 2019, 40(11):235-245. | |
[4] | 黄晓静, 王少敏, 等. 曲霉属真菌毒素的毒性研究进展[J]. 食品安全质量检测学报, 2017, 8(5):1679-1687. |
Huang XJ, Wang SM, et al. Research progress on toxicity of Asper-gillus mycotoxins[J]. J Food Safe Qual, 2017, 8(5):1679-1687. | |
[5] | 刘英丽, 毛慧佳, 刘慧琳, 等. 黄曲霉毒素B1生物法脱毒机制及产物毒性评价[J]. 食品工业科技, 2018, 39(3):324-329. |
Liu YL, Mao HJ, Liu HL, et al. Progress in biological detoxification of aflatoxin B1 and its degradation metabolites toxicity evaluation[J]. Sci Technol Food Ind, 2018, 39(3):324-329. | |
[6] | 蔡俊, 田尔诺, 邵帅, 等. 黄曲霉毒素B1生物脱毒的研究进展[J]. 微生物学通报, 2017, 44(3):726-731. |
Cai J, Tian EN, Shao S, et al. Progress in biological detoxification of Aflatoxin B1[J]. Microbiol China, 2017, 44(3):726-731. | |
[7] | 邵帅, 戴军, 杜馨, 等. 降解黄曲霉毒素B1菌株的发酵条件优化及降解机制[J]. 食品科学, 2016, 37(5):138-143. |
Shao S, Dai J, Du X, et al. Optimization of fermentation condition for an AFB1-degrading strain and preliminary exploration of degradation mechanism[J]. Food Sci, 2016, 37(5):138-143. | |
[8] |
Samuel MS, Sivaramakrishna A, Mehta A. Degradation and detoxification of aflatoxin B1 by Pseudomonas putida[J]. Int Biodeterior Biodegrad, 2014, 86:202-209.
doi: 10.1016/j.ibiod.2013.08.026 URL |
[9] | Eshelli M, Harvey L, Edrada-Ebel R, et al. Metabolomics of the bio-degradation process of aflatoxin B1 by actinomycetes at an initial pH of 6.0[J]. Toxins:Basel, 2015, 7(2):439-456. |
[10] | 张晓雪. 诱变黑曲霉及其固定化对黄曲霉毒素B1的脱毒作用及安全性评价[D]. 无锡:江南大学, 2015. |
Zhang XX. Aflatoxin B1 detoxification and assessment of safety by UV-mutated live and immobilized Aspergillus niger[D]. Wuxi:Jiangnan University, 2015. | |
[11] | 唐璎, 孟宪刚, 等. 西北酸菜中吸附黄曲霉毒素B1乳酸菌株的筛选鉴定及稳定性研究[J]. 食品与发酵工业, 2020, 46(15):60-65. |
Tang Y, Meng XG, et al. Screening and stability of an aflatoxin B1-adsorbing lactic acid bacterium isolated from pickled vegetable in northwest China[J]. Food Ferment Ind, 2020, 46(15):60-65. | |
[12] |
Fochesato AS, Cuello D, Poloni V, et al. Aflatoxin B1 adsorption/desorption dynamics in the presence of Lactobacillus rhamnosus RC007 in a gastrointestinal tract-simulated model[J]. J Appl Microbiol, 2019, 126(1):223-229.
doi: 10.1111/jam.14101 pmid: 30188600 |
[13] | 李文明. 黄曲霉毒素B1生物降解产物的分离鉴定及其致突变性研究[D]. 南昌:南昌大学, 2013. |
Li WM. Separation and characteristics of the products derived from biodegraded aflatoxin B1[D]. Nanchang:Nanchang University, 2013. | |
[14] | 杨登辉, 王鹤达, 等. 核磁共振氢谱法测定食用油中的脂肪酸含量[J]. 河南工业大学学报:自然科学版, 2019, 40(5):13-17. |
Yang DH, Wang HD, et al. Determination of fatty acid contents in edible oils by 1H nuclear magnetic resonance technology[J]. J Henan Univ Technol:Nat Sci Ed, 2019, 40(5):13-17. | |
[15] |
Shu X, et al. Biological degradation of aflatoxin B1 by cell-free extracts of Bacillus velezensis DY3108 with broad PH stability and excellent thermostability[J]. Toxins, 2018, 10(8):330.
doi: 10.3390/toxins10080330 URL |
[16] | 徐明芳, 耿梦梦. 黄曲霉毒素的细胞毒性及机制[J]. 乳业科学与技术, 2018, 41(3):1-9. |
Xu MF, Geng MM. Cytotoxicity and mechanism of aflatoxin[J]. J Dairy Sci Technol, 2018, 41(3):1-9. | |
[17] |
Cheon MJ, et al. Probiotic properties and neuroprotective effects of Lactobacillus buchneri KU200793 isolated from Korean fermented foods[J]. Int J Mol Sci, 2020, 21(4):1227.
doi: 10.3390/ijms21041227 URL |
[18] | 赵春霞, 王轶, 等. 黄曲霉毒素的生物降解研究进展[J]. 湖北农业科学, 2016, 55(20):5172-5176. |
Zhao CX, Wang Y, et al. The research progress of aflatoxins biodegradation[J]. Hubei Agric Sci, 2016, 55(20):5172-5176. | |
[19] |
Raksha Rao K, Vipin AV, Hariprasad P, et al. Biological detoxification of aflatoxin b1 by Bacillus licheniformis CFR1[J]. Food Control, 2017, 71:234-241.
doi: 10.1016/j.foodcont.2016.06.040 URL |
[20] | 张丹枫, 喻国聪. 乙烯-甲基丙烯酸甲酯共聚物结构的1H NMR和13C NMR表征[J]. 分析测试学报, 2018, 37(6):734-738. |
Zhang DF, Yu GC. Characterization on copolymer structure of ethylene and methyl methacrylate by 1H NMR and 13C NMR[J]. J Instrum Anal, 2018, 37(6):734-738. | |
[21] | 于丽娜, 王明清, 等. 黄曲霉毒素B1降解菌株的筛选及鉴定研究[J]. 食品研究与开发, 2018, 39(21):167-171. |
Yu LN, et al. Screening and identification of aflatoxin B1 degradation strains[J]. Food Res Dev, 2018, 39(21):167-171. | |
[22] | 李俊霞, 等. 黄曲霉毒素B1降解菌株的筛选及鉴定[J]. 中国农业科学, 2008, 41(5):1459-1463. |
Li JX, et al. Screening and identification of aflatoxin B1 degradation strains[J]. Sci Agric Sin, 2008, 41(5):1459-1463. | |
[23] | 刘大岭, 姚冬生, 陈敏峰. 真菌提取液对黄曲霉毒素解毒作用的研究[J]. 广东药学院学报, 1995, 11(2):92-94. |
Liu DL, Yao DS, Chen MF. Study on detoxification of aflatoxin B1 by the crude extract of fungi E-2[J]. Acad J Guangdong Coll Pharm, 1995, 11(2):92-94. | |
[24] |
Detroy RW, Hesseltine CW. Aflatoxicol:structure of a new transformation product of aflatoxin B1[J]. Can J Biochem, 1970, 48(7):830-832.
pmid: 4326919 |
[25] |
Alberts JF, Engelbrecht Y, Steyn PS, et al. Biological degradation of aflatoxin B1 by Rhodococcus erythropolis cultures[J]. Int J Food Microbiol, 2006, 109(1/2):121-126.
doi: 10.1016/j.ijfoodmicro.2006.01.019 URL |
[26] | Mann R, Rehm HJ. Degradation products from aflatoxin B1 by By rubrum, Aspergillus niger, Trichoderma viride and Mucor ambiguus[J]. Eur J Appl Microbiol Biotechnol, 1976, 2(4):297-306. |
[27] |
Wang CQ, Li ZY, Wang H, et al. Rapid biodegradation of aflatoxin B1 by metabolites of Fusarium sp. WCQ3361 with broad working temperature range and excellent thermostability[J]. J Sci Food Agric, 2017, 97(4):1342-1348.
doi: 10.1002/jsfa.2017.97.issue-4 URL |
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