Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (1): 278-288.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0167
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WU Xin-yuan(), WANG Guang-chao, LIN Jin-xing, JING Yan-ping()
Received:
2021-02-08
Online:
2022-01-26
Published:
2022-02-22
Contact:
JING Yan-ping
E-mail:wxywuxinyuan@163.com;ypjing@bjfu.edu.cn
WU Xin-yuan, WANG Guang-chao, LIN Jin-xing, JING Yan-ping. Correlative Light and Electron Microscopy and Its Application in Botanical Research[J]. Biotechnology Bulletin, 2022, 38(1): 278-288.
种类 Category | 名称 Name | 特点 Features | 参考文献 Reference |
---|---|---|---|
真空中稳定的荧光探针 Stable fluorescent probes under vacuum | 荧光染料:DAPI、Alex等 Fluorochromes:DAPI,Alexa,etc. | 电子对比度低,电镜下观察不到 | [ |
无机双对比探针 Inorganic dual-contrast probes | 荧光纳米金 Fluoronanogold | 渗透性好,在光镜和电镜下均可直接成像 | [ |
量子点 Quantum dots | 具有高电子密度、良好的光稳定性与抗漂白性,可同时进行不同组分的标记,在光镜和电镜中均可直接成像 | [ | |
基因编码的探针 Genetically encoded probes | 双砷染料-四半胱氨酸系统 Tetracysteinebiarsenical | 目的蛋白连接四半胱氨酸序列,双砷荧光团与之结合而具有强荧光,同时其对DAB具有光转化作用,也可实现在电镜下成像 | [ |
小型单线态氧气发生器 Mini singlet oxygen generator | 分子量比GFP小,在蓝光激发下能够发出绿色荧光,通过氧化DAB形成电镜下可见的嗜锇小体 | [ | |
增强型抗坏血酸过氧化酶 Enhanced ascorbate peroxidase | 当APEX与荧光蛋白结合时,可在活细胞中表达出荧光信号,经过化学固定以及添加DAB和H2O2,可以产生EM可见的反应产物 | [ | |
绿色荧光蛋白及其衍生物 Green fluorescent protein and its derivatives | 需要通过DAB进行光电转化;也可通过高压冷冻与GMA包埋或者Tokuyasu保护荧光蛋白的荧光,实现光电关联 | [ | |
基因编码的化学诱导探针 Genetically-encoded chemically-inducible probes | 荧光重组电子致密的铁蛋白颗粒与感兴趣的蛋白连接,雷帕霉素诱导表达,直接在光镜与电镜下成像 | [ | |
光转换荧光蛋白mEos Photoconvertible fluorescent protein mEos | 电镜制样后,超薄切片中仍保持荧光活性 | [ |
Table 1 CLEM probes
种类 Category | 名称 Name | 特点 Features | 参考文献 Reference |
---|---|---|---|
真空中稳定的荧光探针 Stable fluorescent probes under vacuum | 荧光染料:DAPI、Alex等 Fluorochromes:DAPI,Alexa,etc. | 电子对比度低,电镜下观察不到 | [ |
无机双对比探针 Inorganic dual-contrast probes | 荧光纳米金 Fluoronanogold | 渗透性好,在光镜和电镜下均可直接成像 | [ |
量子点 Quantum dots | 具有高电子密度、良好的光稳定性与抗漂白性,可同时进行不同组分的标记,在光镜和电镜中均可直接成像 | [ | |
基因编码的探针 Genetically encoded probes | 双砷染料-四半胱氨酸系统 Tetracysteinebiarsenical | 目的蛋白连接四半胱氨酸序列,双砷荧光团与之结合而具有强荧光,同时其对DAB具有光转化作用,也可实现在电镜下成像 | [ |
小型单线态氧气发生器 Mini singlet oxygen generator | 分子量比GFP小,在蓝光激发下能够发出绿色荧光,通过氧化DAB形成电镜下可见的嗜锇小体 | [ | |
增强型抗坏血酸过氧化酶 Enhanced ascorbate peroxidase | 当APEX与荧光蛋白结合时,可在活细胞中表达出荧光信号,经过化学固定以及添加DAB和H2O2,可以产生EM可见的反应产物 | [ | |
绿色荧光蛋白及其衍生物 Green fluorescent protein and its derivatives | 需要通过DAB进行光电转化;也可通过高压冷冻与GMA包埋或者Tokuyasu保护荧光蛋白的荧光,实现光电关联 | [ | |
基因编码的化学诱导探针 Genetically-encoded chemically-inducible probes | 荧光重组电子致密的铁蛋白颗粒与感兴趣的蛋白连接,雷帕霉素诱导表达,直接在光镜与电镜下成像 | [ | |
光转换荧光蛋白mEos Photoconvertible fluorescent protein mEos | 电镜制样后,超薄切片中仍保持荧光活性 | [ |
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