Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (1): 77-85.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0181

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Interactions Between ZmJAZ and ZmMYC2 Using Bimolecular Fluorescence Complementation Assay

LV Di(), CHEN Ru-mei, ZHOU Xiao-jin()   

  1. Institute of Biotechnology,Chinese Academy of Agricultural Sciences,Beijing 100081
  • Received:2021-02-13 Online:2022-01-26 Published:2022-02-22
  • Contact: ZHOU Xiao-jin E-mail:lvdi1@foxmail.com;zhouxiaojin@caas.cn

Abstract:

JAZ(Jasmonate ZIM-domain)proteins are negative regulators in the JA signaling pathway,clarifying the interaction between JAZ protein and MYC2 is crucial for understanding the JA signaling pathway. qRT-PCR was applied to examine the expressions of ZmJAZ genes,and discovered seed preferentially expressed ZmJAZ4,reproductive organ predominantly expressed ZmJAZ8,and constitutively expressed ZmJAZ12. Then,the subcellular localizations of these AZ proteins and ZmMYC2 were investigated using protoplasts of maize mesophyll cells transiently expressing vector. Besides,the interactions between these JAZ proteins and ZmMYC2 were analyzed by bimolecular fluorescence complementation(BiFC). The results showed that ZmJAZ4,ZmJAZ8,and ZmJAZ12 were located in cell nucleus,and BiFC assay revealed that these JAZ proteins interacted with ZmMYC2 in cell nucleus,confirming that ZmJAZ family proteins had function of binding key transcription factor ZmMYC2 in JA signal pathway,suggesting that ZmMYC2 can regulate its transcriptional activity by binding to ZmMYC2.

Key words: jasmonic acids, JAZ protein, MYC2 transcription factor, bimolecular fluorescence complementation